The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells

Reactive oxygen species (ROS) regulate various functions of cells, including cell death, viability, and differentiation, and nanoparticles influence ROS depending on their size and shape. Selenium is known to regulate various physiological functions, such as cell differentiations and anti-inflammato...

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Published in:Nanomaterials (Basel, Switzerland) Switzerland), 2021-02, Vol.11 (2), p.557
Main Authors: Lee, Sang-Cheol, Lee, Na-Hyun, Patel, Kapil D, Jang, Tae-Su, Knowles, Jonathan Campbell, Kim, Hae-Won, Lee, Hae-Hyoung, Lee, Jung-Hwan
Format: Article
Language:English
Subjects:
Alizarin
Alkaline phosphatase
Antioxidants
Apoptosis
Aqueous solutions
Biomedical materials
Bone marrow
Catalytic oxidation
Cell cycle
Cell death
Cell differentiation
Cytotoxicity
Differentiation (biology)
Fabrication
Fourier transforms
Gene expression
Hydrogen peroxide
Inflammation
Kinases
MC3T3-E1
Nanomaterials
Nanoparticles
osteogenic differentiation
Osteoporosis
Oxidative stress
Physiology
Reactive oxygen species
Selenium
Staining
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Summary:Reactive oxygen species (ROS) regulate various functions of cells, including cell death, viability, and differentiation, and nanoparticles influence ROS depending on their size and shape. Selenium is known to regulate various physiological functions, such as cell differentiations and anti-inflammatory functions, and plays an important role in the regulation of ROS as an antioxidant. This study aims to investigate the effect of selenium nanoparticles (SeNPs) on the differentiation of osteogenic MC3T3-E1 cells. After fabrication of SeNPs with a size of 25.3 ± 2.6 nm, and confirmation of its oxidase-like activity, SeNPs were added to MC3T3-E1 cells with or without H O : 5~20 μg/mL SeNPs recovered cells damaged by 200 μM H O via the intracellular ROS downregulating role of SeNPs, revealed by the ROS staining assay. The increase in osteogenic maturation with SeNPs was gradually investigated by expression of osteogenic genes at 3 and 7 days, Alkaline phosphatase activity staining at 14 days, and Alizarin red S staining at 28 days. Therefore, the role of SeNPs in regulating ROS and their therapeutic effects on the differentiation of MC3T3-E1 cells were determined, leading to possible applications for bone treatment.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano11020557