Detection of Intracytoplasmic Cytokines by Flow Cytometry

Flow cytometry has been used as a powerful technique for studying cell surface antigen expression as well as intracellular molecules. Its capability of analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2000-06, Vol.95 (3), p.401-402
Main Authors: Santiago, M A, Luca, P M, Bertho, A L, Azeredo-Coutinho, R B, Coutinho, S G
Format: Article
Language:English
Subjects:
Animals
Cytokines - analysis
Cytoplasm - chemistry
flow cytometry
Flow Cytometry - methods
Humans
intracellular cytokines
intracellular cytokines, flow cytometry, leishmaniasis
Leishmania braziliensis
leishmaniasis
Leishmaniasis, Cutaneous - immunology
PARASITOLOGY
TROPICAL MEDICINE
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Summary:Flow cytometry has been used as a powerful technique for studying cell surface antigen expression as well as intracellular molecules. Its capability of analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous populations. In this respect, several techniques have been developed during the past few years to study cytokine-producing cells by flow cytometry in humans and several animal models.
ISSN:1678-8060
0074-0276
0074-0276
1678-8060
DOI:10.1590/S0074-02762000000300017