Preparation, characterization, and in ovo vaccination of dextran-spermine nanoparticle DNA vaccine coexpressing the fusion and hemagglutinin genes against Newcastle disease

• Published in: International journal of nanomedicine 2016-01, Vol.11 (Issue 1), p.259-267
• Main Authors: Firouzamandi, Masoumeh, Moeini, Hassan, Hosseini, Seyed Davood, Bejo, Mohd Hair, Omar, Abdul Rahman, Mehrbod, Parvaneh, El Zowalaty, Mohamed E, Webster, Thomas J, Ideris, Aini
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Viral - immunology; Chick Embryo; Chickens; Deoxyribonucleic acid; Dextran-spermine nanoparticles; Dextrans; Dextrans - chemistry; DNA; DNA vaccine; Embryos; Female; Genes; Hemagglutination Inhibition Tests; Hemagglutinins - administration & dosage; Hemagglutinins - genetics; Hemagglutinins - metabolism; in ovo; Lectins; Medical research; Microscopy; Nanoparticles; Nanoparticles - chemistry; Nanotechnology; Newcastle disease; Newcastle Disease - genetics; Newcastle Disease - immunology; Newcastle Disease - prevention & control; Newcastle disease virus; Newcastle disease virus - immunology; Original Research; Ovum - immunology; Paramyxoviridae; Particle size; Polyamines; Spermine - chemistry; Sunscreening agents; Vaccination; Vaccines; Vaccines, DNA - administration & dosage; Vaccines, DNA - immunology; Veterinary medicine; Viral Vaccines - administration & dosage
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S92225

Plasmid DNA (pDNA)-based vaccines have emerged as effective subunit vaccines against viral and bacterial pathogens. In this study, a DNA vaccine, namely plasmid internal ribosome entry site-HN/F, was applied in ovo against Newcastle disease (ND). Vaccination was carried out using the DNA vaccine alone or as a mixture of the pDNA and dextran-spermine (D-SPM), a nanoparticle used for pDNA delivery. The results showed that in ovo vaccination with 40 μg pDNA/egg alone induced high levels of antibody titer (P0.05). Higher antibody titer was observed in the group immunized with 40 μg pDNA/egg at 4 weeks postvaccination. The findings also showed that vaccination with 40 μg pDNA/egg alone was able to confer protection against Newcastle disease virus strain NDIBS002 in two out of seven SPF chickens. Although the chickens produced antibody titers 3 weeks after in ovo vaccination, it was not sufficient to provide complete protection to the chickens from lethal viral challenge. In addition, vaccination with pDNA/D-SPM complex did not induce high antibody titer when compared with naked pDNA. Therefore, it was concluded that DNA vaccination with plasmid internal ribosome entry site-HN/F can be suitable for in ovo application against ND, whereas D-SPM is not recommended for in ovo gene delivery.