Epidemic of Klebsiella pneumoniae ST11 clone coproducing KPC-2 and 16S rRNA methylase RmtB in a Chinese University Hospital

Emergence of rmtB-positive Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) poses a great threat to antimicrobial treatment options. From January 2010 to December 2010, non-duplicate KPC-KP isolates from our hospital were screened for rmtB and multiple other resistance dete...

Full description

Saved in:
Bibliographic Details
Published in:BMC infectious diseases 2012-12, Vol.12 (1), p.373-373, Article 373
Main Authors: Li, Jun-Jie, Sheng, Zi-Ke, Deng, Mei, Bi, Sheng, Hu, Fei-Shu, Miao, Hai-Feng, Ji, Zhong-Kang, Sheng, Ji-Fang, Li, Lan-Juan
Format: Article
Language:English
Subjects:
Adult
Aged
Aminoglycoside
Analysis
Anti-Bacterial Agents - pharmacology
Antibacterial agents
Antibiotics
Antimicrobial agents
Bacterial pneumonia
beta-Lactamases - genetics
beta-Lactamases - metabolism
Carbapenem
China - epidemiology
Cloning
Cohort Studies
Comparative analysis
Drug resistance
Electrophoresis, Gel, Pulsed-Field
Epidemic
Female
Genetic aspects
Genotype
Hospitalization
Hospitals
Hospitals, University
Humans
Klebsiella
Klebsiella Infections - epidemiology
Klebsiella Infections - microbiology
Klebsiella pneumoniae
Klebsiella pneumoniae - classification
Klebsiella pneumoniae - drug effects
Klebsiella pneumoniae - enzymology
Klebsiella pneumoniae - isolation & purification
KPC
Male
Methyltransferases - genetics
Methyltransferases - metabolism
Microbial Sensitivity Tests
Middle Aged
Mortality
Multilocus Sequence Typing
Neurosurgery
Pneumonia
Polymerase Chain Reaction
Retrospective Studies
rmtB
RNA
Studies
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Emergence of rmtB-positive Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) poses a great threat to antimicrobial treatment options. From January 2010 to December 2010, non-duplicate KPC-KP isolates from our hospital were screened for rmtB and multiple other resistance determinants with PCR. Subsequent studies included MIC determination, PFGE, and multilocus sequence typing. Records from patients with KPC-KP isolated were retrospectively reviewed. Comparisons of molecular and clinical characteristics between rmtB-positive and rmtB-negative isolates were systematically performed, as well as the environmental colonization study in ICU wards. A total of 84 KPC-KP strains were collected, including 48 rmtB-positive KPC-KP (RPKP) and 36 rmtB-negative KPC-KP (RNKP) isolates. All KPC-KP isolates were multidrug resistant, with colistin and tigecycline being the most active agents. Compared with RNKP, RPKP displayed a much severer resistance phenotype. Susceptibility rates for amikacin (0% for RPKP versus 88.9% for RNKP, p 
ISSN:1471-2334
1471-2334
DOI:10.1186/1471-2334-12-373