Repairing chronic myocardial infarction with autologous mesenchymal stem cells engineered tissue in rat promotes angiogenesis and limits ventricular remodeling

• Published in: Journal of biomedical science 2012-11, Vol.19 (1), p.93-93
• Main Authors: Maureira, Pablo, Marie, Pierre-Yves, Yu, Fengxu, Poussier, Sylvain, Liu, Yihua, Groubatch, Frederique, Falanga, Aude, Tran, Nguyen
• Format: Article
• Language: English
• Subjects: Animals; Bone marrow; Cardiology and cardiovascular system; Cell culture; Cells, Cultured; Chronic illnesses; Chronic myocardial infarction; Collagen; Disease Models, Animal; Heart attack; Heart attacks; Heart Ventricles - physiopathology; Human health and pathology; Laboratory animals; Life Sciences; Mesenchymal stem cell; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells - cytology; Myocardial Infarction - physiopathology; Myocardial Infarction - therapy; Neovascularization, Pathologic; Ostomy; Rats; Rodents; SPECT imaging; Stem cells; Tissue Engineering; Transplantation, Autologous; Ventricular Remodeling; Ventriculoplasty
• ISSN: 1423-0127; 1021-7770; 1423-0127
• DOI: 10.1186/1423-0127-19-93

Tissue engineering scaffold constitutes a new strategy of myocardial repair. Here, we studied the contribution of a patch using autologous mesenchymal stem cells (MSCs) seeded on collagen-1 scaffold on the cardiac reconstruction in rat model of chronic myocardial infarction (MI). Patches were cultured with controlled MSCs (growth, phenotype and potentiality). Twenty coronary ligated rats with tomoscingraphy (SPECT)-authenticated transmural chronic MI were referred into a control group (n = 10) and a treated group (n = 10) which beneficiated an epicardial MSC-patch engraftment. Contribution of MSC-patch was tested 1-mo after using non-invasive SPECT cardiac imaging, invasive hemodynamic assessment and immunohistochemistry. 3D-collagen environment affected the cell growth but not the cell phenotype and potentiality. MSC-patch integrates well the epicardial side of chronic MI scar. In treated rats, one-month SPECT data have documented an improvement of perfusion in MI segments compared to control (64 ± 4% vs 49 ± 3% p = 0.02) and a reduced infarction. Contractile parameter dp/dtmax and dp/dtmin were improved (p & 0.01). Histology showed an increase of ventricular wall thickness (1.75 ± 0.24 vs 1.35 ± 0.32 mm, p &0.05) and immunochemistry of the repaired tissue displayed enhanced angiogenesis and myofibroblast-like tissue. 3D-MSC-collagen epicardial patch engraftment contributes to reverse remodeling of chronic MI.