Stem cell regionalization during olfactory bulb neurogenesis depends on regulatory interactions between Vax1 and Pax6

Different subtypes of interneurons, destined for the olfactory bulb, are continuously generated by neural stem cells located in the ventricular and subventricular zones along the lateral forebrain ventricles of mice. Neuronal identity in the olfactory bulb depends on the existence of defined microdo...

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Bibliographic Details
Published in:eLife 2020-08, Vol.9
Main Authors: Coré, Nathalie, Erni, Andrea, Hoffmann, Hanne M, Mellon, Pamela L, Saurin, Andrew J, Beclin, Christophe, Cremer, Harold
Format: Article
Language:English
Subjects:
Animals
Brain
Calbindin
Calbindins - genetics
Cell Differentiation
Developmental Biology
Dopamine receptors
Female
Forebrain
Gene expression
Gene Expression Regulation
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Interneurons
Life Sciences
Male
Mice
MicroRNA
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Molecular modelling
Neural stem cells
Neural Stem Cells - classification
Neural Stem Cells - physiology
Neurogenesis
Neurons
Neuropeptides - genetics
Neuropeptides - metabolism
Neuroscience
Olfactory bulb
Olfactory Bulb - physiology
olfactory bulb interneurons
Pax6 protein
PAX6 Transcription Factor - genetics
PAX6 Transcription Factor - metabolism
postnatal neurogenesis
regulatory interactions
Short Report
Stem cell transplantation
Stem cells
transcription factors
Ventricle
Ventricles (cerebral)
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Summary:Different subtypes of interneurons, destined for the olfactory bulb, are continuously generated by neural stem cells located in the ventricular and subventricular zones along the lateral forebrain ventricles of mice. Neuronal identity in the olfactory bulb depends on the existence of defined microdomains of pre-determined neural stem cells along the ventricle walls. The molecular mechanisms underlying positional identity of these neural stem cells are poorly understood. Here, we show that the transcription factor Vax1 controls the production of two specific neuronal subtypes. First, it is directly necessary to generate Calbindin expressing interneurons from ventro-lateral progenitors. Second, it represses the generation of dopaminergic neurons by dorsolateral progenitors through inhibition of Pax6 expression. We present data indicating that this repression occurs, at least in part, via activation of microRNA miR-7.
ISSN:2050-084X
2050-084X
DOI:10.7554/ELIFE.58215