Rapid Identification of Mycobacterium tuberculosis Complex Using Mass Spectrometry: A Proof of Concept

Mycobacteria that form the complex are responsible for deadly tuberculosis in animals and patients. Identification of these pathogens at the species level is of primary importance for treatment and source tracing and currently relies on DNA analysis, including whole genome sequencing (WGS), which re...

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Bibliographic Details
Published in:Frontiers in microbiology 2022-03, Vol.13, p.753969-753969
Main Authors: Robinne, Simon, Saad, Jamal, Morsli, Madjid, Hamidou, Zelika Harouna, Tazerart, Fatah, Drancourt, Michel, Baron, Sophie Alexandra
Format: Article
Language:English
Subjects:
Bacteriology
Cardiology and cardiovascular system
Emerging diseases
Human health and pathology
identification
Infectious diseases
Life Sciences
MALDI-TOF mass spectrometry
Microbiology
Microbiology and Parasitology
Mycobacterium
Mycobacterium tuberculosis
Parasitology
tuberculosis
Virology
whole genome sequencing
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Summary:Mycobacteria that form the complex are responsible for deadly tuberculosis in animals and patients. Identification of these pathogens at the species level is of primary importance for treatment and source tracing and currently relies on DNA analysis, including whole genome sequencing (WGS), which requires a whole day. In this study, we report the unprecedented discrimination of complex species using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS), with WGS as the comparative reference standard. In the first step, optimized peptide extraction applied to 36 isolates otherwise identified in five of the 11 complex variants by WGS yielded 139 MALDI-TOF spectra, which were used to identify biomarkers of interest that facilitate differentiation between variants. In a second step, 70/80 (88%) other isolates were correctly classified by an algorithm based on specific peaks. This study is the first to report a MALDI-TOF-MS method for discriminating complex mycobacteria that is easily implemented in clinical microbiology laboratories.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2022.753969