GTP-Binding Protein 1-Like (GTPBP1l) Regulates Vascular Patterning during Zebrafish Development

Genetic regulation of vascular patterning is not fully understood. Here, we report a novel gene, ( ), that regulates vascular development in zebrafish. Amino acid sequence comparison and a phylogenetic study showed that is conserved in vertebrates. mRNA is expressed in the vasculature during embryog...

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Bibliographic Details
Published in:Biomedicines 2022-12, Vol.10 (12), p.3208
Main Authors: Lo, Yi-Hao, Huang, Yi-Shan, Chang, Yu-Chiuan, Hung, Pei-Yu, Wang, Wen-Der, Liu, Wangta, Urade, Ritesh, Wen, Zhi-Hong, Wu, Chang-Yi
Format: Article
Language:English
Subjects:
Amino acid sequence
Angiogenesis
Animal genetic engineering
Apoptosis
Binding proteins
Cell death
Conserved sequence
CVP (caudal vein plexus)
Danio rerio
Diagnostic reagents industry
Embryogenesis
Embryonic development
Embryos
Genes
Genetically modified animals
GTP-binding protein
gtpbp1l
Hybridization
ISV (intersegmental vessel)
mRNA
Pattern formation
Phylogeny
Protein binding
Proteins
RNA
RNA polymerase
Transfer RNA
Transgenic fish
Variance analysis
vascular development
Vascular endothelial growth factor
Zebrafish
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Summary:Genetic regulation of vascular patterning is not fully understood. Here, we report a novel gene, ( ), that regulates vascular development in zebrafish. Amino acid sequence comparison and a phylogenetic study showed that is conserved in vertebrates. mRNA is expressed in the vasculature during embryogenesis. Knockdown of by morpholino impairs the patterning of the intersegmental vessel (ISV) and caudal vein plexus (CVP), indicating the role of in vasculature. Further apoptosis assays and transgenic fish tests suggested that vascular defects in morphants are not due to cell death but are likely caused by the impairment of migration and proliferation. Moreover, the altered expression of vessel markers is consistent with the vascular defects in morphants. Finally, we revealed that is regulated by VEGF/notch and BMP signaling. Collectively, these findings showed that plays a critical role in vascular patterning during zebrafish development.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines10123208