Study on the fluorescence properties of the interaction between felodipine and bovine serum protein

The interaction between felodipine and bovine serum protein was studied, the optimal experimental conditions were selected, and the fluorescence quenching mechanism was discussed. The interaction between filodipine and bovine serum protein was determined by UV spectrophotometry, and the optimal expe...

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Bibliographic Details
Published in:E3S web of conferences 2021-01, Vol.300, p.1023
Main Authors: Li, Shuxian, Bu, Jialuo, Liu, Wenbin, Liu, Cuijuan, Lv, Yuguang
Format: Article
Language:English
Subjects:
bovineserumprotein
Buffer solutions
Cattle
complex
Complex compounds
Felodipine
Fluorescence
fluorescence properties study
Proteins
Quenching
Reaction time
Serum proteins
Spectrophotometry
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Summary:The interaction between felodipine and bovine serum protein was studied, the optimal experimental conditions were selected, and the fluorescence quenching mechanism was discussed. The interaction between filodipine and bovine serum protein was determined by UV spectrophotometry, and the optimal experimental conditions were selected by control variable method. The mechanism of fluorescence quenching in the system was explored by fluorescence spectrophotometry. The fluorescence intensity of the system between felodipine and bovine serum protein was the most obvious under the experimental conditions of buffer solution pH 7.4, felodipine concentration 8.0*10 -4 mol/L, reaction time 30min and 25°C. Static fluorescence quenching caused by the formation of complex compounds.
ISSN:2267-1242
2555-0403
2267-1242
DOI:10.1051/e3sconf/202130001023