Altered endothelial dysfunction-related miRs in plasma from ME/CFS patients

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex disease characterized by unexplained debilitating fatigue. Although the etiology is unknown, evidence supports immunological abnormalities, such as persistent inflammation and immune-cell activation, in a subset of patients. Si...

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Bibliographic Details
Published in:Scientific reports 2021-05, Vol.11 (1), p.10604-10604, Article 10604
Main Authors: Blauensteiner, J., Bertinat, R., León, L. E., Riederer, M., Sepúlveda, N., Westermeier, F.
Format: Article
Language:English
Subjects:
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Bioinformatics
Biomarkers
Cell activation
Chronic fatigue syndrome
Epigenetics
Etiology
Fatigue
Histone deacetylase
Homeostasis
Humanities and Social Sciences
Inflammation
Leukocytes (mononuclear)
miRNA
multidisciplinary
Nitric oxide
Nitric-oxide synthase
Oxidative stress
Peripheral blood mononuclear cells
Science
Science (multidisciplinary)
SIRT1 protein
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Summary:Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex disease characterized by unexplained debilitating fatigue. Although the etiology is unknown, evidence supports immunological abnormalities, such as persistent inflammation and immune-cell activation, in a subset of patients. Since the interplay between inflammation and vascular alterations is well-established in other diseases, endothelial dysfunction has emerged as another player in ME/CFS pathogenesis. Endothelial nitric oxide synthase (eNOS) generates nitric oxide (NO) that maintains endothelial homeostasis. eNOS is activated by silent information regulator 1 (Sirt1), an anti-inflammatory protein. Despite its relevance, no study has addressed the Sirt1/eNOS axis in ME/CFS. The interest in circulating microRNAs (miRs) as potential biomarkers in ME/CFS has increased in recent years. Accordingly, we analyze a set of miRs reported to modulate the Sirt1/eNOS axis using plasma from ME/CFS patients. Our results show that miR-21, miR-34a, miR-92a, miR-126, and miR-200c are jointly increased in ME/CFS patients compared to healthy controls. A similar finding was obtained when analyzing public miR data on peripheral blood mononuclear cells. Bioinformatics analysis shows that endothelial function-related signaling pathways are associated with these miRs, including oxidative stress and oxygen regulation. Interestingly, histone deacetylase 1, a protein responsible for epigenetic regulations, represented the most relevant node within the network. In conclusion, our study provides a basis to find endothelial dysfunction-related biomarkers and explore novel targets in ME/CFS.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-89834-9