SPINK4 promotes colorectal cancer cell proliferation and inhibits ferroptosis

Little is known about the role of serine peptidase inhibitor Kazal type 4 (SPINK4) in colorectal cancer (CRC) and ferroptosis. Therefore, this study aimed to determine the effect of SPINK4 on CRC pathogenesis and ferroptosis. SPINK4 expression was analyzed in public datasets and examined using immun...

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Bibliographic Details
Published in:BMC gastroenterology 2023-04, Vol.23 (1), p.104-104, Article 104
Main Authors: Hu, Bang-Li, Yin, Yi-Xin, Li, Ke-Zhi, Li, Si-Qi, Li, Zhao
Format: Article
Language:English
Subjects:
Analysis
Animal models
Animals
Antibodies
Apoptosis
Cancer therapies
Care and treatment
Cell culture
Cell growth
Cell Line
Cell Line, Tumor
Cell Proliferation
Chemotherapy
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - pathology
Datasets
Development and progression
Experiments
Ferroptosis
Gastroenterology
Gene expression
Genetic aspects
Health aspects
Immunofluorescence
Immunohistochemistry
Medical research
Metabolism
Metastases
Metastasis
Mice
Mouse model
mRNA
Prevention
Risk factors
Serine peptidase
Serine Peptidase Inhibitors, Kazal Type - genetics
Serine Peptidase Inhibitors, Kazal Type - metabolism
SPINK4
Statistical analysis
Tumors
Variance analysis
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Summary:Little is known about the role of serine peptidase inhibitor Kazal type 4 (SPINK4) in colorectal cancer (CRC) and ferroptosis. Therefore, this study aimed to determine the effect of SPINK4 on CRC pathogenesis and ferroptosis. SPINK4 expression was analyzed in public datasets and examined using immunohistochemistry. The biological function of SPINK4 in CRC cell lines and its effect on ferroptosis were tested. An immunofluorescence assay was performed to determine the location of SPINK4 in cells, and mouse models were established to determine the effects of SPINK4 in vivo. CRC datasets and clinical samples analysis revealed that SPINK4 mRNA and protein levels were significantly reduced in CRC tissues compared to control tissues (P 
ISSN:1471-230X
1471-230X
DOI:10.1186/s12876-023-02734-2