miR-8b is involved in brain and eye regeneration of Dugesia japonica in head regeneration

MicroRNAs (miRNAs) are a class of evolutionarily conserved small non-coding RNAs that regulate gene expression at the translation level in cell growth, proliferation and differentiation. In addition, some types of miRNAs have been proven to be key modulators of both CNS development and plasticity, s...

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Bibliographic Details
Published in:Biology open 2021-06, Vol.10 (6)
Main Authors: Liu, Hongjin, Song, Qian, Zhen, Hui, Deng, Hongkuan, Zhao, Bosheng, Cao, Zhonghong
Format: Article
Language:English
Subjects:
Amputation
Animals
Brain
brain regeneration
Brain research
Cell differentiation
Cell growth
Central nervous system
Developmental plasticity
Dugesia japonica
Evolutionary conservation
Eye
Eyespot
Gene expression
Gene Expression Regulation
Gene Knockdown Techniques
Head
Insects
Ligands
MicroRNAs
MicroRNAs - genetics
mir-8b
miRNA
Modulators
Nervous system
Non-coding RNA
Phenotype
Photoreceptors
planarian
Planarians - physiology
Regeneration
Transcription factors
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Summary:MicroRNAs (miRNAs) are a class of evolutionarily conserved small non-coding RNAs that regulate gene expression at the translation level in cell growth, proliferation and differentiation. In addition, some types of miRNAs have been proven to be key modulators of both CNS development and plasticity, such as let-7, miR-9 and miR-124. In this research, we found miR-8b acts as an important regulator involved in brain and eyespot regeneration in Dugesia japonica. miR-8b was highly conserved among species and was abundantly expressed in central nervous system. Here, we detected the expression dynamics of miR-8b by qPCR during the head regeneration of D. japonica. Knockdown miR-8b by anti-MIRs method caused severe defects of eyes and CNS. Our study revealed the evolutionary conserved role of miR-8b in the planarian regeneration process, and further provided more research ideas and available information for planarian miRNAs.
ISSN:2046-6390
2046-6390
DOI:10.1242/bio.058538