Differences in endosomal Rab gene expression between positive and negative COVID-19 patients

SARS CoV-2, the etiologic agent of coronavirus disease-2019 (COVID-19) is well-known to use ACE2 to begin internalization. Some viruses enter the host cell through the endocytosis process and involve some endocytosis proteins, such as the Rab family. However, the relationship between SARS CoV-2 infe...

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Bibliographic Details
Published in:BMC research notes 2022-07, Vol.15 (1), p.1-252, Article 252
Main Authors: Atik, Nur, Wirawan, Farruqi, Amalia, Riezki, Khairani, Astrid Feinisa, Pradini, Gita Widya
Format: Article
Language:English
Subjects:
ACE2
Angiotensin-converting enzyme 2
Comparative analysis
Coronaviruses
COVID-19
DNA polymerase
Endocytic pathway
Endocytosis
Gene expression
Genomes
Internalization
Proteins
Rab11b
Rab5
Rab7
Research Note
SARS CoV-2
Severe acute respiratory syndrome coronavirus 2
Statistical analysis
Thermal cycling
Viral entry
Viruses
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Summary:SARS CoV-2, the etiologic agent of coronavirus disease-2019 (COVID-19) is well-known to use ACE2 to begin internalization. Some viruses enter the host cell through the endocytosis process and involve some endocytosis proteins, such as the Rab family. However, the relationship between SARS CoV-2 infection with endocytic mRNA RAB5, RAB7, and RAB11B is unknown. This study aims to compare the expression of RAB5, RAB7, and RAB11B between positive and negative COVID-19 patient groups. Both viral and human epithelial RNA Isolation and RT-PCR were performed from 249 samples. The genes expression was analysed using appropriate statistical tests. We found the Median (inter-quartile range/IQR) of RAB5, RAB7, and RAB11B expression among the COVID-19 patient group was 2.99 (1.88), 0.17 (0.47), 0.47 (1.49), and 1.60 (2.88), 1.05 (2.49), 1.10 (3.96) among control group respectively. We proceeded with Mann Whitney U Test and found that RAB5 expression was significantly increased (P < 0.001), and RAB7 and RAB11B expression was significantly decreased (P < 0.001 and P = 0.036) in the COVID-19 patient group compared to the control group. This first report showed significant differences in RAB5, RAB7, and RAB11B exist between COVID-19 positive and negative patients.
ISSN:1756-0500
1756-0500
DOI:10.1186/s13104-022-06144-7