Enzymatic Defense Response of Apple Aphid Aphis pomi to Increased Temperature

• Published in: Insects (Basel, Switzerland) Switzerland), 2020-07, Vol.11 (7), p.436
• Main Authors: Dampc, Jan, Kula-Maximenko, Monika, Molon, Mateusz, Durak, Roma
• Format: Article
• Language: English
• Subjects: aphids; Aphis pomi; Catalase; Cellobiase; Climate change; Controlled conditions; Environmental stress; enzymatic markers; Enzymes; Experiments; Glucosidase; Glutathione; Glutathione transferase; Heat; Heat stress; Heat tolerance; Humidity; Insects; Metabolic rate; Metabolism; Metabolites; Neutralization; Organisms; Oxidative stress; Peroxidase; Pollution monitoring; Polyphenol oxidase; Proteins; Reactive oxygen species; Respiration; Superoxide dismutase; Temperature; Temperature effects; β-Glucosidase
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects11070436

Climate change, and in particular the increase in temperature we are currently observing, can affect herbivorous insects. Aphids, as poikilothermic organisms, are directly exposed to temperature increases that influence their metabolism. Heat stress causes disturbances between the generations and the neutralization of reactive oxygen species (ROS). The aim of this work is focused on explaining how the aphid, using the example of , responds to abiotic stress caused by temperature increase. The experiment was carried out under controlled conditions at three temperatures: 20, 25, and 28 °C. In the first stage, changes in the activity of enzymatic markers (superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), β-glucosidase, polyphenol oxidase (PPO), and peroxidase (POD)) were determined in aphid tissues, at each temperature. In the second stage, microcalorimetry monitored changes in heat emitted by aphids, at each temperature. Our results showed that defense responses varied depending on temperature and were highest at 28 °C. The flexible activity of enzymes and increase in the metabolic rate played the role of adaptive mechanisms and ran more effectively at higher temperatures. The thus protected itself against ROS excessive induction and the aphids were able to respond quickly to environmental stress.