Taurine reduces ER stress in C. elegans

• Published in: Journal of biomedical science 2010-08, Vol.17 Suppl 1 (Suppl 1), p.S26-S26
• Main Authors: Kim, Hye Min, Do, Chang-Hee, Lee, Dong Hee
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Caenorhabditis elegans - anatomy & histology; Caenorhabditis elegans - drug effects; Caenorhabditis elegans - physiology; Caenorhabditis elegans Proteins - metabolism; DNA-Binding Proteins - metabolism; Dose-Response Relationship, Drug; Endoplasmic Reticulum - drug effects; Endoplasmic Reticulum - physiology; HSP70 Heat-Shock Proteins - metabolism; Life Expectancy; Review; Stress, Physiological - drug effects; Taurine - pharmacology; Transcription Factors - metabolism; Tunicamycin - pharmacology
• ISSN: 1423-0127; 1021-7770; 1423-0127
• DOI: 10.1186/1423-0127-17-S1-S26

ER stress is a strong indicator of whether or not a cell is undergoing physiological stress. C. elegans is a practical system of characterizing the effect of ER stress at the in vivo or organismal level. This study characterized taurine's anti-ER stress potential employing western blotting on ER stress markers and assays of motility, lifespan comparison, and fecundity measurement. When treated with tunicamycin, C. elegans showed the typical ER stress symptoms. It showed a higher expression of hsp-70 and skn-1 than the non-treated control. Survivorship significantly decreased under tunicamycin treatment, and the offspring number also decreased. During the synchronized culture under ER stress conditions, the C. elegans showed early signs of aging especially between L3 and L4 within their life span, along with lowered motility. The worms, however, showed a positive response to the taurine treatment under ER stress conditions. When C. elegans were treated with taurine before or after the tunicamycin treatment, they showed a less severe level of ER stress, including an enhanced survivorship, increased motility, and augmented fecundity. Taken together, these results strongly indicate that taurine works positively to cope with ER stress from the organismal perspective.