In vivo laser speckle contrast imaging of 4-aminopyridine- or pentylenetetrazole-induced seizures

• Published in: APL bioengineering 2023-09, Vol.7 (3), p.036119-036119
• Main Authors: Wang, Yuhling, Tsytsarev, Vassiliy, Liao, Lun-De
• Format: Article
• Language: English
• ISSN: 2473-2877; 2473-2877
• DOI: 10.1063/5.0158791

Clinical and preclinical studies on epileptic seizures are closely linked to the study of neurovascular coupling. Obtaining reliable information about cerebral blood flow (CBF) in the area of epileptic activity through minimally invasive techniques is crucial for research in this field. In our studies, we used laser speckle contrast imaging (LSCI) to gather information about the local blood circulation in the area of epileptic activity. We used two models of epileptic seizures: one based on 4-aminopyridine (4-AP) and another based on pentylenetetrazole (PTZ). We verified the duration of an epileptic seizure using electrocorticography (ECoG). We applied the antiepileptic drug topiramate (TPM) to both models, but its effect was different in each case. However, in both models, TPM had an effect on neurovascular coupling in the area of epileptic activity, as shown by both LSCI and ECoG data. We demonstrated that TPM significantly reduced the amplitude of 4-AP-induced epileptic seizures (4-AP+TPM: 0.61 ± 0.13 mV vs 4-AP: 1.08 ± 0.19 mV; p