Applicability of Hyaluronic Acid-Alginate Hydrogel and Ovarian Cells for In Vitro Development of Mouse Preantral Follicles

In the present study, the applicability of hyaluronic acid-alginate (HAA) hydrogel and ovarian cells (OCs) for the culture of mouse ovarian follicles were investigated and compared with those of alginate (ALG) and fibrin-alginate (FA) hydrogels. In the first step of this experimental study, mechanic...

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Bibliographic Details
Published in:Cell journal (Yakhteh) 2020-09, Vol.22 (Suppl 1), p.49-60
Main Authors: Jamalzaei, Parisa, Rezazadeh Valojerdi, Mojtaba, Montazeri, Leila, Baharvand, Hossein
Format: Article
Language:English
Subjects:
17β-Estradiol
alginate
Alginates
Alginic acid
Androstenedione
Cell culture
Encapsulation
Fibrin
Follicles
Gametocytes
Hyaluronic acid
Hydrogels
Maturation
Meiosis
Metaphase
Oocytes
Original
ovarian cells
Ovaries
preantral follicle
Progesterone
Secretions
Sex hormones
Survival
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Summary:In the present study, the applicability of hyaluronic acid-alginate (HAA) hydrogel and ovarian cells (OCs) for the culture of mouse ovarian follicles were investigated and compared with those of alginate (ALG) and fibrin-alginate (FA) hydrogels. In the first step of this experimental study, mechanically isolated preantral follicles from the ovaries of two-week-old mice were encapsulated in the absence or presence of OCs in ALG, HAA, and FA hydrogels and cultured for 14 days. The morphology, diameter, survival and antrum formation rates of the follicles and the maturation and quality of the oocytes were evaluated during culture. In the second step, preantral follicles were cultured similar to the first step, but for 13 days, and their gene expressions and hormonal secretion were assessed on the last day of culture. In the absence of OCs, higher numbers of ALG- and HAA-encapsulated follicles reached the antral stage compared to FA-encapsulated follicles (P
ISSN:2228-5806
2228-5814
DOI:10.22074/cellj.2020.6925