Early Extracellular ATP Signaling in Arabidopsis Root Epidermis: A Multi-Conductance Process

Adenosine 5'-triphosphate (ATP) is an important extracellular signaling agent, operating in growth regulation, stomata! conductance, and wound response. With the first receptor for extracellular ATP now identified in plants (P2K1/DORN1) and a plasma membrane NADPH oxidase revealed as its target...

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Bibliographic Details
Published in:Frontiers in plant science 2019-09, Vol.10, p.1064-1064
Main Authors: Wang, Limin, Stacey, Gary, Leblanc-Fournier, Nathalie, Legué, Valérie, Moulia, Bruno, Davies, Julia M.
Format: Article
Language:English
Subjects:
anion
ATP
channel
DORN1
Life Sciences
P2K1
Plant Science
root epidermis
Vegetal Biology
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Summary:Adenosine 5'-triphosphate (ATP) is an important extracellular signaling agent, operating in growth regulation, stomata! conductance, and wound response. With the first receptor for extracellular ATP now identified in plants (P2K1/DORN1) and a plasma membrane NADPH oxidase revealed as its target, the search continues for the components of the signaling cascades they command. The Arabidopsis root elongation zone epidermal plasma membrane has recently been shown to contain cation transport pathways (channel conductances) that operate downstream of P2K1 and could contribute to extracellular ATP (eATP) signaling. Here, patch clamp electrophysiology has been used to delineate two further conductances from the root elongation zone epidermal plasma membrane that respond to eATP, including one that would permit chloride transport. This perspective addresses how these conductances compare to those previously characterized in roots and how they might operate together to enable early events in eATP signaling, including elevation of cytosolic-free calcium as a second messenger. The role of the reactive oxygen species (ROS) that could arise from eATP's activation of NADPH oxidases is considered in a qualitative model that also considers the regulation of plasma membrane potential by the concerted action of the various cation and anion conductances. The molecular identities of the channel conductances in eATP signaling remain enigmatic but may yet be found in the multigene families of glutamate receptor-like channels, cyclic nucleotide-gated channels, annexins, and aluminum-activated malate transporters.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2019.01064