Campylobacter jejuni increases the paracellular permeability of broiler chickens in a dose-dependent manner

In recent years, several studies emphasize the deleterious effects of Campylobacter jejuni on the chicken intestine. In this context, it was shown that C. jejuni, contrary to the general belief, has a negative influence on the gut barrier in chickens. More precisely, we demonstrated that C. jejuni a...

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Bibliographic Details
Published in:Poultry science 2020-11, Vol.99 (11), p.5407-5414
Main Authors: Awad, Wageha A., Ruhnau, Daniel, Hess, Claudia, Hess, Michael
Format: Article
Language:English
Subjects:
Animals
bacterial dose
broiler chickens
Campylobacter Infections - immunology
Campylobacter Infections - veterinary
Campylobacter jejuni
Cell Membrane Permeability - immunology
Chickens
colonization
Female
Immunology, Health and Disease
Intestinal Mucosa - cytology
Intestinal Mucosa - immunology
Intestinal Mucosa - microbiology
Male
permeability
Poultry Diseases - immunology
Poultry Diseases - microbiology
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Summary:In recent years, several studies emphasize the deleterious effects of Campylobacter jejuni on the chicken intestine. In this context, it was shown that C. jejuni, contrary to the general belief, has a negative influence on the gut barrier in chickens. More precisely, we demonstrated that C. jejuni affects gut physiology characterized by changes in ion transport and transepithelial ion conductance, but the underlying mechanism is yet to be investigated. In the actual study, to determine epithelial paracellular permeability, the mucosal to serosal flux of 14C-mannitol in the small and large intestine was measured applying Ussing chamber. A total of seventy-five 1-day-old Ross 308 broiler chickens were housed in floor pens on wood shavings with feed and water provided ad libitum. Birds were randomly allocated to 3 different groups (n = 25 with 5 replicates/group) and infected at 14 d of age with a high (108 colony forming units [CFU]) or a low (104 CFU) dose of C. jejuni and a third group kept as noninfected control. Infection with the low dose of C. jejuni resulted in delayed cecal colonization but equalized at 21 d postinfection, independent of the dose. Invasion of liver and spleen with C. jejuni was only noticed in birds infected with 108 (CFU). Body weight (BW) and body weight gain of all birds infected with C. jejuni were lower than in the control group and varied with the dose of infection, confirming a negative correlation between the infection dose and birds BW. Mannitol flux in jejunum and cecum was significantly (P 
ISSN:0032-5791
1525-3171
1525-3171
DOI:10.1016/j.psj.2020.08.014