New Insights Into Biomphalysin Gene Family Diversification in the Vector Snail Biomphalaria glabrata

Aerolysins initially characterized as virulence factors in bacteria are increasingly found in massive genome and transcriptome sequencing data from metazoans. Horizontal gene transfer has been demonstrated as the main way of aerolysin-related toxins acquisition in metazoans. However, only few studie...

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Bibliographic Details
Published in:Frontiers in immunology 2021-04, Vol.12, p.635131-635131
Main Authors: Pinaud, Silvain, Tetreau, Guillaume, Poteaux, Pierre, Galinier, Richard, Chaparro, Cristian, Lassalle, Damien, Portet, Anaïs, Simphor, Elodie, Gourbal, Benjamin, Duval, David
Format: Article
Language:English
Subjects:
aerolysin
Biodiversity
biomphalaria
biomphalysin
Immunology
invertebrate immunity
Life Sciences
Populations and Evolution
pore-forming toxin (PFT)
structure
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Summary:Aerolysins initially characterized as virulence factors in bacteria are increasingly found in massive genome and transcriptome sequencing data from metazoans. Horizontal gene transfer has been demonstrated as the main way of aerolysin-related toxins acquisition in metazoans. However, only few studies have focused on their potential biological functions in such organisms. Herein, we present an extensive characterization of a multigene family encoding aerolysins - named biomphalysin - in snail, the intermediate host of the trematode . Our results highlight that duplication and domestication of an acquired bacterial toxin gene in the snail genome result in the acquisition of a novel and diversified toxin family. Twenty-three biomphalysin genes were identified. All are expressed and exhibited a tissue-specific expression pattern. An structural analysis was performed to highlight the central role played by two distinct domains i) a large lobe involved in the lytic function of these snail toxins which constrained their evolution and ii) a small lobe which is structurally variable between biomphalysin toxins and that matched to various functional domains involved in moiety recognition of targets cells. A functional approach suggests that the repertoire of biomphalysins that bind to pathogens, depends on the type of pathogen encountered. These results underline a neo-and sub-functionalization of the biomphalysin toxins, which have the potential to increase the range of effectors in the snail's immune arsenal.
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2021.635131