Specific Proteomic Identification of Collagen-Binding Proteins in Escherichia coli O157:H7: Characterisation of OmpA as a Potent Vaccine Antigen

• Published in: Cells (Basel, Switzerland) Switzerland), 2023-06, Vol.12 (12), p.1634
• Main Authors: Monteiro, Ricardo, Chafsey, Ingrid, Caccia, Nelly, Ageorges, Valentin, Leroy, Sabine, Viala, Didier, Hébraud, Michel, Livrelli, Valérie, Pizza, Mariagrazia, Pezzicoli, Alfredo, Desvaux, Mickaël
• Format: Article
• Language: English
• Subjects: Animals; Antibiotic resistance; Antigens; Bacteria; Bacterial antigens; Carrier Proteins; cell adhesion; cell surface protein; Collagen; Control; E coli; Epithelial cells; Escherichia coli; Escherichia coli O157 - genetics; Escherichia coli Proteins - genetics; Extracellular matrix; extracellular matrix (ECM); Genetic aspects; Genomes; host cell-bacteria interaction; Identification and classification; Immunogenicity; Infections; intestinal epithelial cell; Intestine; Life Sciences; Pathogenesis; Phenotypes; Physiological aspects; Proteins; Proteomics; Public health; Strains (organisms); Vaccines; Virulence; Virulence factors
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells12121634

is a versatile commensal species of the animal gut that can also be a pathogen able to cause intestinal and extraintestinal infections. The plasticity of its genome has led to the evolution of pathogenic strains, which represent a threat to global health. Additionally, strains are major drivers of antibiotic resistance, highlighting the urgent need for new treatment and prevention measures. The antigenic and structural heterogeneity of enterohaemorrhagic colonisation factors has limited their use for the development of effective and cross-protective vaccines. However, the emergence of new strains that express virulence factors deriving from different diarrhoeagenic pathotypes suggests that a vaccine targeting conserved proteins could be a more effective approach. In this study, we conducted proteomics analysis and functional protein characterisation to identify a group of proteins potentially involved in the adhesion of O157:H7 to the extracellular matrix and intestinal epithelial cells. Among them, OmpA has been identified as a highly conserved and immunogenic antigen, playing a significant role in the adhesion phenotype of O157:H7 and in bacterial aggregation. Furthermore, antibodies raised against recombinant OmpA effectively reduced the adhesion of O157:H7 to intestinal epithelial cells. The present work highlights the role of OmpA as a potent antigen for the development of a vaccine against intestinal pathogenic .