Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in Xenopus

Marcks and Marcksl1 are abundant proteins that shuttle between the cytoplasm and membrane to modulate multiple cellular processes, including cytoskeletal dynamics, proliferation, and secretion. Here, we performed loss- and gain-of-function experiments in to reveal the novel roles of these proteins i...

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Bibliographic Details
Published in:eLife 2024-12, Vol.13
Main Authors: El Amri, Mohamed, Pandit, Abhay, Schlosser, Gerhard
Format: Article
Language:English
Subjects:
Animals
axon outgrowth
development
Developmental Biology
Marcks
Membrane Proteins - genetics
Membrane Proteins - metabolism
Myristoylated Alanine-Rich C Kinase Substrate - metabolism
neurogenesis
Physiological aspects
Regeneration
Spinal cord
Spinal Cord - embryology
Spinal Cord - metabolism
Spinal Cord Regeneration
Xenopus
Xenopus laevis
Xenopus Proteins - genetics
Xenopus Proteins - metabolism
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Summary:Marcks and Marcksl1 are abundant proteins that shuttle between the cytoplasm and membrane to modulate multiple cellular processes, including cytoskeletal dynamics, proliferation, and secretion. Here, we performed loss- and gain-of-function experiments in to reveal the novel roles of these proteins in spinal cord development and regeneration. We show that Marcks and Marcksl1 have partly redundant functions and are required for normal neurite formation and proliferation of neuro-glial progenitors during embryonic spinal cord development and for its regeneration during tadpole stages. Rescue experiments in Marcks and Marcksl1 loss-of-function animals further suggested that some of the functions of Marcks and Marcksl1 in the spinal cord are mediated by phospholipid signaling. Taken together, these findings identify Marcks and Marcksl1 as critical new players in spinal cord development and regeneration and suggest new pathways to be targeted for therapeutic stimulation of spinal cord regeneration in human patients.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.98277