Insights into the cotranscriptional and translational control mechanisms of the Escherichia coli tbpA thiamin pyrophosphate riboswitch

• Published in: Communications biology 2024-10, Vol.7 (1), p.1345-10, Article 1345
• Main Authors: Grondin, Jonathan P., Geffroy, Mélanie, Simoneau-Roy, Maxime, Chauvier, Adrien, Turcotte, Pierre, St-Pierre, Patrick, Dubé, Audrey, Moreau, Julie, Massé, Eric, Penedo, J. Carlos, Lafontaine, Daniel A.
• Format: Article
• Language: English
• Subjects: 38/71; 631/45/535; 631/57/2265; Aptamers; Biomedical and Life Sciences; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Gene expression; Gene Expression Regulation, Bacterial; Gene regulation; Genetic control; Life Sciences; Nucleic Acid Conformation; Post-transcription; Protein Biosynthesis; Riboswitch - genetics; Riboswitches; RNA, Bacterial - chemistry; RNA, Bacterial - genetics; RNA, Bacterial - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Thiamine; Thiamine diphosphate; Thiamine Pyrophosphate - metabolism; Transcription initiation; Transcription, Genetic; Translation initiation
• ISSN: 2399-3642; 2399-3642
• DOI: 10.1038/s42003-024-07008-5

Riboswitches regulate gene expression by modulating their structure upon metabolite binding. These RNA orchestrate several layers of regulation to achieve genetic control. Although Escherichia coli riboswitches modulate translation initiation, several cases have been reported where riboswitches also modulate mRNA levels. Here, we characterize the regulation mechanisms of the thiamin pyrophosphate (TPP) tbpA riboswitch in E. coli . Our results indicate that the tbpA riboswitch modulates both levels of translation and transcription and that TPP sensing is achieved more efficiently cotranscriptionally than post-transcriptionally. The preference for cotranscriptional binding is also observed when monitoring the TPP-dependent inhibition of translation initiation. Using single-molecule approaches, we observe that the aptamer domain freely fluctuates between two main structures involved in TPP recognition. Our results suggest that translation initiation is controlled through the ligand-dependent stabilization of the riboswitch structure. This study demonstrates that riboswitch cotranscriptional sensing is the primary determinant in controlling translation and mRNA levels. In vivo and in vitro studies of an Escherichia coli riboswitch highlights the role of cotranscriptional metabolite sensing and the mechanism of gene regulation.