Beauveria bassiana interacts with gut and hemocytes to manipulate Aedes aegypti immunity

Mosquito-borne diseases affect millions of people. Chemical insecticides are currently employed against mosquitoes. However, many cases of insecticide resistance have been reported. Entomopathogenic fungi (EPF) have demonstrated potential as a bioinsecticide. Here, we assessed the invasion of the EP...

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Bibliographic Details
Published in:Parasites & vectors 2023-01, Vol.16 (1), p.17-17, Article 17
Main Authors: de Oliveira Barbosa Bitencourt, Ricardo, Corrêa, Thaís Almeida, Santos-Mallet, Jacenir, Santos, Huarrisson Azevedo, Lowenberger, Carl, Moreira, Haika Victória Sales, Gôlo, Patrícia Silva, Bittencourt, Vânia Rita Elias Pinheiro, da Costa Angelo, Isabele
Format: Article
Language:English
Subjects:
Aedes
Aedes - microbiology
Aedes albopictus
Animals
Antimicrobial peptides
Ascomycota
Beauveria
Biological control
Entomopathogenic fungi
Fungi
Health aspects
Hemocytes
Humans
Immune response
Immune system
Insect pests
Insecticides
Larva - microbiology
Microscopy, Electron, Scanning
Mosquito
Observations
Pest Control, Biological - methods
Pesticide resistance
Spores, Fungal
Virulence (Microbiology)
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Summary:Mosquito-borne diseases affect millions of people. Chemical insecticides are currently employed against mosquitoes. However, many cases of insecticide resistance have been reported. Entomopathogenic fungi (EPF) have demonstrated potential as a bioinsecticide. Here, we assessed the invasion of the EPF Beauveria bassiana into Aedes aegypti larvae and changes in the activity of phenoloxidase (PO) as a proxy for the general activation of the insect innate immune system. In addition, other cellular and humoral responses were evaluated. Larvae were exposed to blastospores or conidia of B. bassiana CG 206. After 24 and 48 h, scanning electron microscopy (SEM) was conducted on the larvae. The hemolymph was collected to determine changes in total hemocyte concentration (THC), the dynamics of hemocytes, and to observe hemocyte-fungus interactions. In addition, the larvae were macerated to assess the activity of PO using L-DOPA conversion, and the expression of antimicrobial peptides (AMPs) was measured using quantitative Real-Time PCR. Propagules invaded mosquitoes through the midgut, and blastopores were detected inside the hemocoel. Both propagules decreased the THC regardless of the time. By 24 h after exposure to conidia the percentage of granulocytes and oenocytoids increased while the prohemocytes decreased. By 48 h, the oenocytoid percentage increased significantly (P 
ISSN:1756-3305
1756-3305
DOI:10.1186/s13071-023-05655-x