Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi

Sequences of the complete nuclear ribosomal DNA (rDNA) gene from five individual Paramphistomum cervi were determined for the first time. The five complete rDNA sequences, which included the 18S rDNA, the internal transcribed spacer 1 (ITS1), the 5.8S rDNA, the internal transcribed spacer 2 (ITS2),...

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Published in:TheScientificWorld 2014-01, Vol.2014 (2014), p.1-11
Main Authors: Zhu, Xing-Quan, Wang, Chun-Ren, Duan, Hong, Lou, Yan, Tian, Si-Qin, Zhang, Yan, Chang, Qiao-Cheng, Zheng, Xu, Guo, Dong-Hui
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Digenea
DNA, Helminth - chemistry
DNA, Ribosomal - chemistry
Evolution
Genes
Molecular Sequence Data
Nucleotide sequence
Paramphistomatidae
Paramphistomatidae - genetics
Paramphistomum cervi
Parasites
Phylogenetics
Phylogeny
Science
Sequence Alignment
Sequence Analysis, DNA
Studies
Systematics
Zoological research
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Summary:Sequences of the complete nuclear ribosomal DNA (rDNA) gene from five individual Paramphistomum cervi were determined for the first time. The five complete rDNA sequences, which included the 18S rDNA, the internal transcribed spacer 1 (ITS1), the 5.8S rDNA, the internal transcribed spacer 2 (ITS2), the 28S rDNA, and the intergenic spacer (IGS) regions, had a length range of 8,493–10,221 bp. The lengths of the investigated 18S, ITS1, 5.8S, ITS2, and 28S rDNA sequences, which were 1,994 bp, 1,293 bp, 157 bp, 286 bp, and 4,186 bp, respectively, did not vary. However, the IGS rDNA sequences had a length range of 577–2,305 bp. The 5.8S and ITS-2 rDNA sequences had 100% identity among the five investigated samples, while the identities among the IGS had a range of 53.7–99.8%. A comparative analysis revealed that different types and numbers of repeats were found within each ITS1 and IGS region, which may be related to the length polymorphism of IGS. The phylogenetic position of P. cervi in Paramphistomatidae was analyzed based on the 18S rDNA sequences. These results will aid in studying the intra- and interspecific variation of the Paramphistomatidae and the systematics and phylogenetics of Digenea.
ISSN:2356-6140
1537-744X
1537-744X
DOI:10.1155/2014/751907