Misidentification of prostamides as prostaglandins

Prostaglandins and endogenous cannabinoid metabolites share the same lipid backbone with differing polar head groups at exactly the position through which a large molecule is attached to provide antigenicity and thus raise antisera. Hence, we hypothesized that antisera raised against prostaglandins...

Full description

Saved in:
Bibliographic Details
Published in:Journal of lipid research 2005-07, Vol.46 (7), p.1364-1368
Main Authors: Glass, Michelle, Hong, Jiwon, Sato, Timothy A, Mitchell, Murray D
Format: Article
Language:English
Subjects:
Amnion - cytology
anandamide
Arachidonic Acids - metabolism
Cannabinoid Receptor Modulators - immunology
Cells, Cultured
Chromatography, Thin Layer - methods
Cross Reactions
Cyclooxygenase 2
Dinoprostone - analogs & derivatives
Dinoprostone - biosynthesis
Dinoprostone - immunology
Endocannabinoids
ethanolamides
Female
Humans
Interleukin-1 - pharmacology
Membrane Proteins
Polyunsaturated Alkamides
Prostaglandin-Endoperoxide Synthases - biosynthesis
Prostaglandins - immunology
prostamides
Radioimmunoassay
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Prostaglandins and endogenous cannabinoid metabolites share the same lipid backbone with differing polar head groups at exactly the position through which a large molecule is attached to provide antigenicity and thus raise antisera. Hence, we hypothesized that antisera raised against prostaglandins linked to a large molecule such as BSA at the carboxyl functional group would also recognize endogenous cannabinoid metabolites and lead to highly misleading interpretations of data. We found major cross-reactivity of commercial antisera raised to prostaglandins with endocannabinoid metabolites. Furthermore, in a well-characterized cell line (WISH) or primary amnion tissue explants, endocannabinoid treatment led to increased production of endocannabinoid metabolites as opposed to primary prostaglandins. This was apparent only after separation of products by thin-layer chromatography, because they measured as prostaglandins by radioimmunoassay. These findings have major implications for our interpretation of data in situations in which these prostaglandin-like molecules are formed, and they stress the need for chromatographic or spectrometric confirmation of prostaglandin production detected by antibody-based methods.
ISSN:0022-2275
1539-7262
DOI:10.1194/jlr.C500006-JLR200