Nanotechnology in Wound Healing; Semisolid Dosage Forms Containing Curcumin-Ampicillin Solid Lipid Nanoparticles, in-Vitro, Ex-Vivo and in-Vivo Characteristics

• Published in: Advanced pharmaceutical bulletin 2018-08, Vol.8 (3), p.395-400
• Main Authors: Ghaffari, Solmaz, Alihosseini, Faezeh, Rezayat Sorkhabadi, Seyed Mahdi, Arbabi Bidgoli, Sepideh, Mousavi, Seyyedeh Elaheh, Haghighat, Setareh, Afshar Nasab, Ahoo, Kianvash, Nooshin
• Format: Article
• Language: English
• Subjects: Animals; Antibiotics; Drug dosages; Homogenization; In-vivo; Lipids; Morphology; Nanotechnology; Olive oil; Particle size; Penicillin; Semi solids; Skin; Solid Lipid Nanoparticles; Studies; Wound healing
• ISSN: 2228-5881; 2251-7308
• DOI: 10.15171/apb.2018.046

Researchers demonstrated that nanofibers showed effectiveness in skin regeneration based on their structures which are similar to the extracellular matrix.4 Numerous innovative nanoscale products have emerged for wound healing that are currently under clinical investigation.5 Nash and Hugh demonstrated that topical ampicillin could help to reduce the incidence of infections after cancer operation of the large intestine also topical ampicillin showed a significant reduction in the frequency of infections after operation for cancer of the colon and rectum and side effects were not reported as well.6 Based on our knowledge there is no publication in which curcumin and ampicillin nanoparticles together apply via semi solid formulation for burn wound healing, in the present research we tried to evaluate the benefit of this rout of administration. Animal's manipulation for creating skin burn model Adult male Wistar rats weighing 200-230 g were obtained from Animal house of Experimental Medicine Research Center of Tehran University of Medical Sciences. Inflammation, collagen deposition, angiogenesis, granulation tissue formation and epithelialization were graded as 0-3 to each section.10 The burn wounds surface area, were photographed at the 0th, 5th, 11th, 14th, 18th and 21th days using a digital camera; then, the healing rate was evaluated by comparison of wound area at each control days with the wound area in the first day.11 Results and Discussion Particle size and morphology evaluation of nanoparticles SEM photographs demonstrated that most of the particles are spherical even after freeze drying and non-significant size enlargement was seen after freeze drying. Curcumin was encapsulated into a silanehydrogel nanoparticle vehicle (curc-np) to overcome native curcumin's poor solubility and investigate its potential as a topical therapy for wound infection by Krausz et al.17 Curc-np demonstrated efficacy in vitro against methicillin-resistant Staphylococcus aureus and inhibited growth of this bacteria strain and enhanced wound healing in an in vivo burn wound model.