Acinetobacter baumannii infections in Amazon Region driven by extensively drug resistant international clones, 2016-2018

BACKGROUNDAcinetobacter baumannii is a leading cause of nosocomial infections. This species is characterised by the presence of pandemic lineages (International Clones) that present a broad antimicrobial resistance profile. OBJECTIVETo perform the molecular epidemiology of carbapenem-resistant A. ba...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2019-01, Vol.114, p.e190232-e190232
Main Authors: Caldart, Raquel Vosges, Fonseca, Erica L, Freitas, Fernanda, Rocha, Luiza, Vicente, Ana Carolina
Format: Article
Language:English
Subjects:
Acinetobacter baumannii
Amazon Region
bla OXA-23
extensively drug resistance
international clone
Original
PARASITOLOGY
TROPICAL MEDICINE
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Summary:BACKGROUNDAcinetobacter baumannii is a leading cause of nosocomial infections. This species is characterised by the presence of pandemic lineages (International Clones) that present a broad antimicrobial resistance profile. OBJECTIVETo perform the molecular epidemiology of carbapenem-resistant A. baumannii from a clinical setting in the Amazon Basin, and to characterise their antimicrobial resistance determinants. METHODSThe genetic relationship of carbapenem-resistant A. baumannii were assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Class A, B and D β-lactamase genes were screened by polymerase chain reaction (PCR) and sequencing. The antimicrobial susceptibility profile was obtained by Disc-diffusion method and minimum inhibitory concentration (MIC) determination. FINDINGSAll carbapenem-resistant A. baumannii strains belonged to three international clones, IC-1, IC-5 and IC-6, the latter recently reported by the first time in Brazil. The major determinant of carbapenem resistance in IC-1 and IC-5 strains was bla OXA-23, associated with ISAba1 and ISAba3, respectively, while IC-6 harboured the bla OXA-72. CONCLUSIONSThe A. baumannii epidemiology in Brazilian Amazon Region was unknown. It was demonstrated that A. baumannii XDR international clones were responsible for nosocomial infections in Boa Vista during 2016-2018, revealing that the epidemiological scenario of A. baumannii infections in Amazon Region resembles those from the cosmopolitan regions worldwide.
ISSN:0074-0276
1678-8060
1678-8060
DOI:10.1590/0074-02760190232