A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand

The Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An. barbirostris, An. c...

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Bibliographic Details
Published in:Parasites & vectors 2019-05, Vol.12 (1), p.223-223, Article 223
Main Authors: Brosseau, Laura, Udom, Chanya, Sukkanon, Chutipong, Chareonviriyaphap, Theeraphap, Bangs, Michael J, Saeung, Atiporn, Manguin, Sylvie
Format: Article
Language:English
Subjects:
Alleles
Animals
Anopheles
Anopheles - classification
Aquatic insects
Barbirostris Complex
Bionomics
Deoxyribonucleic acid
Disease transmission
DNA
DNA Primers
DNA, Ribosomal Spacer - genetics
ecology
Female
Filariasis
Human diseases
Identification
Identification and classification
Indonesia
internal transcribed spacers
ITS2
Life Sciences
Malaria
Microbiology and Parasitology
Morphology
Mosquito Vectors - classification
Mosquitoes
multiplex PCR
Multiplex Polymerase Chain Reaction
Multiplexing
Nucleotide sequence
Parasitology
PCR
Phylogeny
Polymerase chain reaction
Ribosomal DNA
Species
Thailand
Vector-borne diseases
Vectors
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Description
Summary:The Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An. barbirostris, An. campestris, An. dissidens, An. saeungae and An. wejchoochotei, while An. vanderwulpi occurs in Indonesia. As these species cannot be accurately differentiated by morphological characters, there is a crucial lack of information on their bionomics and role in the transmission of malaria and filariasis agents. For differentiating the six species, an allele-specific amplification (AS-PCR) based on the second internal transcribed spacer (ITS2) sequence was developed. From 862 mosquitoes in the Barbirostris Complex collected in 23 provinces throughout Thailand, the AS-PCR was able to identify five species and its validation was undertaken on 185 specimens. This multiplex-PCR assay is potentially able to definitely identify all six species of the Barbirostris Complex and was validated on five species present in Thailand.
ISSN:1756-3305
1756-3305
DOI:10.1186/s13071-019-3494-8