A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand

The Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An. barbirostris, An. c...

Full description

Saved in:
Bibliographic Details
Published in:Parasites & vectors 2019-05, Vol.12 (1), p.223-223, Article 223
Main Authors: Brosseau, Laura, Udom, Chanya, Sukkanon, Chutipong, Chareonviriyaphap, Theeraphap, Bangs, Michael J, Saeung, Atiporn, Manguin, Sylvie
Format: Article
Language:English
Subjects:
Alleles
Animals
Anopheles
Anopheles - classification
Aquatic insects
Barbirostris Complex
Bionomics
Deoxyribonucleic acid
Disease transmission
DNA
DNA Primers
DNA, Ribosomal Spacer - genetics
ecology
Female
Filariasis
Human diseases
Identification
Identification and classification
Indonesia
internal transcribed spacers
ITS2
Life Sciences
Malaria
Microbiology and Parasitology
Morphology
Mosquito Vectors - classification
Mosquitoes
multiplex PCR
Multiplex Polymerase Chain Reaction
Multiplexing
Nucleotide sequence
Parasitology
PCR
Phylogeny
Polymerase chain reaction
Ribosomal DNA
Species
Thailand
Vector-borne diseases
Vectors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c699t-9fdf48cd1788ae40c6cfc2f582dadb6c016dd973a191d012b3ba8c3bf930c31f3
cites cdi_FETCH-LOGICAL-c699t-9fdf48cd1788ae40c6cfc2f582dadb6c016dd973a191d012b3ba8c3bf930c31f3
container_end_page 223
container_issue 1
container_start_page 223
container_title Parasites & vectors
container_volume 12
creator Brosseau, Laura
Udom, Chanya
Sukkanon, Chutipong
Chareonviriyaphap, Theeraphap
Bangs, Michael J
Saeung, Atiporn
Manguin, Sylvie
description The Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An. barbirostris, An. campestris, An. dissidens, An. saeungae and An. wejchoochotei, while An. vanderwulpi occurs in Indonesia. As these species cannot be accurately differentiated by morphological characters, there is a crucial lack of information on their bionomics and role in the transmission of malaria and filariasis agents. For differentiating the six species, an allele-specific amplification (AS-PCR) based on the second internal transcribed spacer (ITS2) sequence was developed. From 862 mosquitoes in the Barbirostris Complex collected in 23 provinces throughout Thailand, the AS-PCR was able to identify five species and its validation was undertaken on 185 specimens. This multiplex-PCR assay is potentially able to definitely identify all six species of the Barbirostris Complex and was validated on five species present in Thailand.
doi_str_mv 10.1186/s13071-019-3494-8
format article
fullrecord <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_e35f7e8d423449e289d9be7ad8996952</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A586510451</galeid><doaj_id>oai_doaj_org_article_e35f7e8d423449e289d9be7ad8996952</doaj_id><sourcerecordid>A586510451</sourcerecordid><originalsourceid>FETCH-LOGICAL-c699t-9fdf48cd1788ae40c6cfc2f582dadb6c016dd973a191d012b3ba8c3bf930c31f3</originalsourceid><addsrcrecordid>eNqNkktv1DAUhSMEoqXwA9igSGxgkeJ37A3SaAR0pJFApawtx48Zj5J4sJNR--9xmqF0ukJZOL7-7knu8SmKtxBcQsjZpwQxqGEFoKgwEaTiz4pzWFNWYQzo80fvZ8WrlHYAMCAoe1mcYQg4p5icF_2i7MZ28PvW3pY_ltelSkndlS7Ectja0hvbD955rQYf-jK40vmDLdPeam_TtJ-oRR_2W9vmQqNi42NIQ_Sp1KG7l_V9ebNVvlW9eV28cKpN9s1xvSh-ff1ys7yq1t-_rZaLdaWZEEMlnHGEawNrzpUlQDPtNHKUI6NMwzSAzBhRYwUFNACiBjeKa9w4gYHG0OGLYjXrmqB2ch99p-KdDMrL-0KIG6ni4HVrpcXU1ZYbgjAhwiIujGhsrQwXggmKstbnWWs_Np01OjsSVXsienrS-63chINkFFIGJ4GPs8D2SdvVYi2nGkAE5NvAB5jZD8ePxfB7tGmQnU_attk8G8YkEaohp4LV7D9QjACi2cKMvn-C7sIY-3wDmUI15oQAkKnLmdqo7IvvXcjj6PwY23kdeut8ri8oz4MBQuG_wY4NmRns7bBRY0py9fP6lIUzq3M8UrTuwQkI5JRmOadZ5jTLKc1y-u13j51_6PgbX_wHbbnuXQ</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2227384400</pqid></control><display><type>article</type><title>A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand</title><source>Open Access: PubMed Central</source><source>Publicly Available Content Database</source><creator>Brosseau, Laura ; Udom, Chanya ; Sukkanon, Chutipong ; Chareonviriyaphap, Theeraphap ; Bangs, Michael J ; Saeung, Atiporn ; Manguin, Sylvie</creator><creatorcontrib>Brosseau, Laura ; Udom, Chanya ; Sukkanon, Chutipong ; Chareonviriyaphap, Theeraphap ; Bangs, Michael J ; Saeung, Atiporn ; Manguin, Sylvie</creatorcontrib><description>The Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An. barbirostris, An. campestris, An. dissidens, An. saeungae and An. wejchoochotei, while An. vanderwulpi occurs in Indonesia. As these species cannot be accurately differentiated by morphological characters, there is a crucial lack of information on their bionomics and role in the transmission of malaria and filariasis agents. For differentiating the six species, an allele-specific amplification (AS-PCR) based on the second internal transcribed spacer (ITS2) sequence was developed. From 862 mosquitoes in the Barbirostris Complex collected in 23 provinces throughout Thailand, the AS-PCR was able to identify five species and its validation was undertaken on 185 specimens. This multiplex-PCR assay is potentially able to definitely identify all six species of the Barbirostris Complex and was validated on five species present in Thailand.</description><identifier>ISSN: 1756-3305</identifier><identifier>EISSN: 1756-3305</identifier><identifier>DOI: 10.1186/s13071-019-3494-8</identifier><identifier>PMID: 31088534</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Alleles ; Animals ; Anopheles ; Anopheles - classification ; Aquatic insects ; Barbirostris Complex ; Bionomics ; Deoxyribonucleic acid ; Disease transmission ; DNA ; DNA Primers ; DNA, Ribosomal Spacer - genetics ; ecology ; Female ; Filariasis ; Human diseases ; Identification ; Identification and classification ; Indonesia ; internal transcribed spacers ; ITS2 ; Life Sciences ; Malaria ; Microbiology and Parasitology ; Morphology ; Mosquito Vectors - classification ; Mosquitoes ; multiplex PCR ; Multiplex Polymerase Chain Reaction ; Multiplexing ; Nucleotide sequence ; Parasitology ; PCR ; Phylogeny ; Polymerase chain reaction ; Ribosomal DNA ; Species ; Thailand ; Vector-borne diseases ; Vectors</subject><ispartof>Parasites &amp; vectors, 2019-05, Vol.12 (1), p.223-223, Article 223</ispartof><rights>COPYRIGHT 2019 BioMed Central Ltd.</rights><rights>2019. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>The Author(s) 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c699t-9fdf48cd1788ae40c6cfc2f582dadb6c016dd973a191d012b3ba8c3bf930c31f3</citedby><cites>FETCH-LOGICAL-c699t-9fdf48cd1788ae40c6cfc2f582dadb6c016dd973a191d012b3ba8c3bf930c31f3</cites><orcidid>0000-0002-4911-2287 ; 0000-0002-5925-7164</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515612/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2227384400?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,25734,27905,27906,36993,36994,44571,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31088534$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.umontpellier.fr/hal-02405343$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Brosseau, Laura</creatorcontrib><creatorcontrib>Udom, Chanya</creatorcontrib><creatorcontrib>Sukkanon, Chutipong</creatorcontrib><creatorcontrib>Chareonviriyaphap, Theeraphap</creatorcontrib><creatorcontrib>Bangs, Michael J</creatorcontrib><creatorcontrib>Saeung, Atiporn</creatorcontrib><creatorcontrib>Manguin, Sylvie</creatorcontrib><title>A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand</title><title>Parasites &amp; vectors</title><addtitle>Parasit Vectors</addtitle><description>The Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An. barbirostris, An. campestris, An. dissidens, An. saeungae and An. wejchoochotei, while An. vanderwulpi occurs in Indonesia. As these species cannot be accurately differentiated by morphological characters, there is a crucial lack of information on their bionomics and role in the transmission of malaria and filariasis agents. For differentiating the six species, an allele-specific amplification (AS-PCR) based on the second internal transcribed spacer (ITS2) sequence was developed. From 862 mosquitoes in the Barbirostris Complex collected in 23 provinces throughout Thailand, the AS-PCR was able to identify five species and its validation was undertaken on 185 specimens. This multiplex-PCR assay is potentially able to definitely identify all six species of the Barbirostris Complex and was validated on five species present in Thailand.</description><subject>Alleles</subject><subject>Animals</subject><subject>Anopheles</subject><subject>Anopheles - classification</subject><subject>Aquatic insects</subject><subject>Barbirostris Complex</subject><subject>Bionomics</subject><subject>Deoxyribonucleic acid</subject><subject>Disease transmission</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>ecology</subject><subject>Female</subject><subject>Filariasis</subject><subject>Human diseases</subject><subject>Identification</subject><subject>Identification and classification</subject><subject>Indonesia</subject><subject>internal transcribed spacers</subject><subject>ITS2</subject><subject>Life Sciences</subject><subject>Malaria</subject><subject>Microbiology and Parasitology</subject><subject>Morphology</subject><subject>Mosquito Vectors - classification</subject><subject>Mosquitoes</subject><subject>multiplex PCR</subject><subject>Multiplex Polymerase Chain Reaction</subject><subject>Multiplexing</subject><subject>Nucleotide sequence</subject><subject>Parasitology</subject><subject>PCR</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Ribosomal DNA</subject><subject>Species</subject><subject>Thailand</subject><subject>Vector-borne diseases</subject><subject>Vectors</subject><issn>1756-3305</issn><issn>1756-3305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNkktv1DAUhSMEoqXwA9igSGxgkeJ37A3SaAR0pJFApawtx48Zj5J4sJNR--9xmqF0ukJZOL7-7knu8SmKtxBcQsjZpwQxqGEFoKgwEaTiz4pzWFNWYQzo80fvZ8WrlHYAMCAoe1mcYQg4p5icF_2i7MZ28PvW3pY_ltelSkndlS7Ectja0hvbD955rQYf-jK40vmDLdPeam_TtJ-oRR_2W9vmQqNi42NIQ_Sp1KG7l_V9ebNVvlW9eV28cKpN9s1xvSh-ff1ys7yq1t-_rZaLdaWZEEMlnHGEawNrzpUlQDPtNHKUI6NMwzSAzBhRYwUFNACiBjeKa9w4gYHG0OGLYjXrmqB2ch99p-KdDMrL-0KIG6ni4HVrpcXU1ZYbgjAhwiIujGhsrQwXggmKstbnWWs_Np01OjsSVXsienrS-63chINkFFIGJ4GPs8D2SdvVYi2nGkAE5NvAB5jZD8ePxfB7tGmQnU_attk8G8YkEaohp4LV7D9QjACi2cKMvn-C7sIY-3wDmUI15oQAkKnLmdqo7IvvXcjj6PwY23kdeut8ri8oz4MBQuG_wY4NmRns7bBRY0py9fP6lIUzq3M8UrTuwQkI5JRmOadZ5jTLKc1y-u13j51_6PgbX_wHbbnuXQ</recordid><startdate>20190514</startdate><enddate>20190514</enddate><creator>Brosseau, Laura</creator><creator>Udom, Chanya</creator><creator>Sukkanon, Chutipong</creator><creator>Chareonviriyaphap, Theeraphap</creator><creator>Bangs, Michael J</creator><creator>Saeung, Atiporn</creator><creator>Manguin, Sylvie</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H95</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-4911-2287</orcidid><orcidid>https://orcid.org/0000-0002-5925-7164</orcidid></search><sort><creationdate>20190514</creationdate><title>A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand</title><author>Brosseau, Laura ; Udom, Chanya ; Sukkanon, Chutipong ; Chareonviriyaphap, Theeraphap ; Bangs, Michael J ; Saeung, Atiporn ; Manguin, Sylvie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c699t-9fdf48cd1788ae40c6cfc2f582dadb6c016dd973a191d012b3ba8c3bf930c31f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Alleles</topic><topic>Animals</topic><topic>Anopheles</topic><topic>Anopheles - classification</topic><topic>Aquatic insects</topic><topic>Barbirostris Complex</topic><topic>Bionomics</topic><topic>Deoxyribonucleic acid</topic><topic>Disease transmission</topic><topic>DNA</topic><topic>DNA Primers</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>ecology</topic><topic>Female</topic><topic>Filariasis</topic><topic>Human diseases</topic><topic>Identification</topic><topic>Identification and classification</topic><topic>Indonesia</topic><topic>internal transcribed spacers</topic><topic>ITS2</topic><topic>Life Sciences</topic><topic>Malaria</topic><topic>Microbiology and Parasitology</topic><topic>Morphology</topic><topic>Mosquito Vectors - classification</topic><topic>Mosquitoes</topic><topic>multiplex PCR</topic><topic>Multiplex Polymerase Chain Reaction</topic><topic>Multiplexing</topic><topic>Nucleotide sequence</topic><topic>Parasitology</topic><topic>PCR</topic><topic>Phylogeny</topic><topic>Polymerase chain reaction</topic><topic>Ribosomal DNA</topic><topic>Species</topic><topic>Thailand</topic><topic>Vector-borne diseases</topic><topic>Vectors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brosseau, Laura</creatorcontrib><creatorcontrib>Udom, Chanya</creatorcontrib><creatorcontrib>Sukkanon, Chutipong</creatorcontrib><creatorcontrib>Chareonviriyaphap, Theeraphap</creatorcontrib><creatorcontrib>Bangs, Michael J</creatorcontrib><creatorcontrib>Saeung, Atiporn</creatorcontrib><creatorcontrib>Manguin, Sylvie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Parasites &amp; vectors</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brosseau, Laura</au><au>Udom, Chanya</au><au>Sukkanon, Chutipong</au><au>Chareonviriyaphap, Theeraphap</au><au>Bangs, Michael J</au><au>Saeung, Atiporn</au><au>Manguin, Sylvie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand</atitle><jtitle>Parasites &amp; vectors</jtitle><addtitle>Parasit Vectors</addtitle><date>2019-05-14</date><risdate>2019</risdate><volume>12</volume><issue>1</issue><spage>223</spage><epage>223</epage><pages>223-223</pages><artnum>223</artnum><issn>1756-3305</issn><eissn>1756-3305</eissn><abstract>The Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An. barbirostris, An. campestris, An. dissidens, An. saeungae and An. wejchoochotei, while An. vanderwulpi occurs in Indonesia. As these species cannot be accurately differentiated by morphological characters, there is a crucial lack of information on their bionomics and role in the transmission of malaria and filariasis agents. For differentiating the six species, an allele-specific amplification (AS-PCR) based on the second internal transcribed spacer (ITS2) sequence was developed. From 862 mosquitoes in the Barbirostris Complex collected in 23 provinces throughout Thailand, the AS-PCR was able to identify five species and its validation was undertaken on 185 specimens. This multiplex-PCR assay is potentially able to definitely identify all six species of the Barbirostris Complex and was validated on five species present in Thailand.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>31088534</pmid><doi>10.1186/s13071-019-3494-8</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-4911-2287</orcidid><orcidid>https://orcid.org/0000-0002-5925-7164</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1756-3305
ispartof Parasites & vectors, 2019-05, Vol.12 (1), p.223-223, Article 223
issn 1756-3305
1756-3305
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_e35f7e8d423449e289d9be7ad8996952
source Open Access: PubMed Central; Publicly Available Content Database
subjects Alleles
Animals
Anopheles
Anopheles - classification
Aquatic insects
Barbirostris Complex
Bionomics
Deoxyribonucleic acid
Disease transmission
DNA
DNA Primers
DNA, Ribosomal Spacer - genetics
ecology
Female
Filariasis
Human diseases
Identification
Identification and classification
Indonesia
internal transcribed spacers
ITS2
Life Sciences
Malaria
Microbiology and Parasitology
Morphology
Mosquito Vectors - classification
Mosquitoes
multiplex PCR
Multiplex Polymerase Chain Reaction
Multiplexing
Nucleotide sequence
Parasitology
PCR
Phylogeny
Polymerase chain reaction
Ribosomal DNA
Species
Thailand
Vector-borne diseases
Vectors
title A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T05%3A31%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20multiplex%20PCR%20assay%20for%20the%20identification%20of%20five%20species%20of%20the%20Anopheles%20barbirostris%20complex%20in%20Thailand&rft.jtitle=Parasites%20&%20vectors&rft.au=Brosseau,%20Laura&rft.date=2019-05-14&rft.volume=12&rft.issue=1&rft.spage=223&rft.epage=223&rft.pages=223-223&rft.artnum=223&rft.issn=1756-3305&rft.eissn=1756-3305&rft_id=info:doi/10.1186/s13071-019-3494-8&rft_dat=%3Cgale_doaj_%3EA586510451%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c699t-9fdf48cd1788ae40c6cfc2f582dadb6c016dd973a191d012b3ba8c3bf930c31f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2227384400&rft_id=info:pmid/31088534&rft_galeid=A586510451&rfr_iscdi=true