Isolation of a Highly Efficient Antigenic-Protein-Degrading Bacillus amyloliquefaciens and Assessment of Its Safety

The aims of this study were to screen and isolate a highly efficient strain from the rumen of a cow that can degrade the antigenic soy proteins in soybean meal (SBM) and improve the nutritional value of SBM by fermenting it with this strain. The safety of this strain was investigated with an acute o...

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Bibliographic Details
Published in:Animals (Basel) 2020-07, Vol.10 (7), p.1144
Main Authors: Li, Yang, Guo, Baozhu, Li, Chong, Wang, Weiwei, Wu, Zhengke, Liu, Guohua, Cai, Huiyi
Format: Article
Language:English
Subjects:
Amino acids
Animals
antigenic protein
B. amyloliquefaciens
Bacillus amyloliquefaciens
Bacteria
Conglycinin
Digestibility
Dry matter
Enzymes
Fermentation
Fermented food
Microorganisms
Nitrogen
Nutrient content
Peptides
Proteins
safety assessment
Solid state
Solid state fermentation
soybean meal
Soybeans
Toxicity
Toxicity testing
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Summary:The aims of this study were to screen and isolate a highly efficient strain from the rumen of a cow that can degrade the antigenic soy proteins in soybean meal (SBM) and improve the nutritional value of SBM by fermenting it with this strain. The safety of this strain was investigated with an acute oral toxicity test. A Bacillus amyloliquefaciens strain was successfully screened with plate tests and fermentation. After solid state fermentation of SBM with B. amyloliquefaciens for 24 h, the amounts of glycinin and β-conglycinin, two major antigenic proteins in SBM, decreased by 92.32% and 85.05%, respectively. The crude protein content in the fermented soybean meal (FSBM) increased by 17.54% compared with that in SBM. Notably, the trichloroacetic-acid-soluble protein (TCA-SP) content, particularly small peptides and free amino acids, was 9.97-fold higher in FSBM than in SBM. The in vitro dry matter digestibility and digestible energy of SBM increased from 62.91% to 72.52% and from 10.42 MJ/kg to 13.37 MJ/kg (dry matter basis), respectively, after fermentation. The acute oral toxicity test suggested that the strain exerted no harmful effects on the relative organ weights, the morphological tissue structure, or the health of mice. These results indicate that the B. amyloliquefaciens strain isolated in this study is a safe strain for animals, and could be used to improve the nutritional quality of SBM by solid-state fermentation.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani10071144