Generation and characterization of human-derived iPSC lines from one pair of dizygotic twins discordant for schizophrenia

Schizophrenia (SCZ) is a debilitating neurodevelopmental disorder with a high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by a pair of dizygotic twins. The female was clinically diagnosed as SCZ by Diagnostic and Statistical Manual of Mental Disorders IV (DSM...

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Published in:Stem cell research 2022-04, Vol.60, p.102710-102710, Article 102710
Main Authors: Ni, Peiyan, Wang, Huiyao, Cai, Jia, Ran, Junzhe, Jiang, Youhui, Zhao, Liansheng, Wei, Jinxue, Ni, Rongjun, Wang, Yingcheng, Ma, Xiaohong, Wang, Qiang, Guo, Wanjun, Li, Tao
Format: Article
Language:English
Subjects:
Cell Differentiation
Dizygotic twins
Female
Humans
Induced Pluripotent Stem Cells
Induced pluripotent stem cells (iPSCs)
Kruppel-Like Factor 4
Leukocytes, Mononuclear
Male
Schizophrenia (SCZ)
Schizophrenia - genetics
Twins, Dizygotic
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Summary:Schizophrenia (SCZ) is a debilitating neurodevelopmental disorder with a high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by a pair of dizygotic twins. The female was clinically diagnosed as SCZ by Diagnostic and Statistical Manual of Mental Disorders IV (DSM-IV) criteria, and her unaffected male sibling was healthy control. Induced pluripotent stem cells (iPSCs) were established using Episomal vectors carrying reprograming factors OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4, and SV40LT. These lines with normal karyotype highly expressed pluripotency markers and are capable to differentiate into derivatives of three germ layers. Both lines are negative of mycoplasma.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2022.102710