Increasing Resistance and Changes in Distribution of Serotypes of Streptococcus agalactiae in Poland

Streptococcus agalactiae is responsible for serious infections in newborn babies, pregnant women, and other patients. The aim of this study was to evaluate antimicrobial susceptibility, serotype distribution, and virulence determinants of the S. agalactiae isolates derived from clinical specimens co...

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Published in:Pathogens (Basel) 2020-06, Vol.9 (7), p.526
Main Authors: Kaminska, Dorota, Ratajczak, Magdalena, Szumała-Kąkol, Anna, Dlugaszewska, Jolanta, Nowak-Malczewska, Dorota M., Gajecka, Marzena
Format: Article
Language:English
Subjects:
Antibiotic resistance
Antibiotics
Antigens
Antiinfectives and antibacterials
Babies
biofilm
Biofilms
Clindamycin
decreased susceptibility to penicillin G
Erythromycin
Evaluation
GBS serotypes
Genes
group B Streptococcus
Infections
macrolide resistance
Minimum inhibitory concentration
Penicillin
Pregnancy
Proteins
Serotypes
Streptococcus agalactiae
Streptococcus infections
Virulence
Virulence factors
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Summary:Streptococcus agalactiae is responsible for serious infections in newborn babies, pregnant women, and other patients. The aim of this study was to evaluate antimicrobial susceptibility, serotype distribution, and virulence determinants of the S. agalactiae isolates derived from clinical specimens considering the global increase of both antibiotic resistance and virulence. A total of 165 isolates were identified and serotyped by PCR techniques. Antimicrobial susceptibility was assessed by disk diffusion method, gradient diffusion method and VITEK® System. Virulence associated genes were investigated by PCR; ability to form biofilm was assessed using a microtiter plate assay. The highest observed MIC value for penicillin G was 0.12 µg/mL, seen in 8.5% of isolates. Resistance to erythromycin and clindamycin were found in 30.38% and 24.8% of the strains, respectively. The serotype III (32.73%), V (25.45%), and Ia (18.18%) were found as the most frequently represented. Previously unidentified strains in Poland, belonging to serotypes VI (three strains) and VII (one strain) were recognized. The presence of genes encoding various virulence factors as well as diverse ability to form biofilm were found. In conclusion, macrolide-resistance and decreased susceptibility to penicillin G were revealed signifying the increasing resistance among group B streptococci. Moreover, the presence of genes encoding various virulence factors and the ability to form biofilm were confirmed indicating their role in the pathomechanisms of the evaluated GBS infections.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens9070526