Differential Induction Pattern Towards Classically Activated Macrophages in Response to an Immunomodulatory Extract from Pleurotus ostreatus Mycelium

mushroom preparations have been investigated because of their ability to modulate the immune function. However, there is still no consensus regarding the activation and polarizing effect on macrophages by -derived bioproducts. This study examined the immune-activating effect of a mycelium-derived aq...

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Bibliographic Details
Published in:Journal of fungi (Basel) 2021-03, Vol.7 (3), p.206
Main Authors: Llauradó Maury, Gabriel, Morris-Quevedo, Humberto J, Heykers, Annick, Lanckacker, Ellen, Cappoen, Davie, Delputte, Peter, Vanden Berghe, Wim, Salgueiro, Zelene, Cos, Paul
Format: Article
Language:English
Subjects:
Antigen presentation
Arginase
Carbohydrates
Cell activation
Cytokines
Cytotoxicity
Gene expression
Immune response
immune-activating
Immunology
Immunomodulation
Innate immunity
Interleukin 6
macrophage
Macrophages
mushroom
Mushrooms
mycelium
NF-κB protein
Nitric oxide
Nitric-oxide synthase
Phagocytes
Pleurotus ostreatus
Resazurin test
Tumor necrosis factor-α
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Summary:mushroom preparations have been investigated because of their ability to modulate the immune function. However, there is still no consensus regarding the activation and polarizing effect on macrophages by -derived bioproducts. This study examined the immune-activating effect of a mycelium-derived aqueous extract (HW-Pm) on macrophage functions, by means of the determination of nitric oxide (NO) production, the mRNA expression of inducible nitric oxide synthase (iNOS), Arginase-1 and FIZZ and the cytokine levels. The phagocytic activity and the activation of NF-κB in U937 reporter cells were also investigated. No cytotoxicity was observed in macrophages treated with HW-Pm (IC > 1024 μg/mL) by the resazurin test. HW-Pm induced high levels of NO production and iNOS expression in macrophages. In contrast, HW-Pm did not induce Arginase-1 and FIZZ mRNA expressions. The mushroom extract increased TNF-α and IL-6 production and the phagocytic function in murine macrophages. It also stimulated the activation of the NF-κB promoter. The mycelium extract has a potential application as a natural immune-enhancing agent, by targeting macrophage activation towards the classically activated subset and stimulating macrophage-mediated innate immune responses.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof7030206