Extraction Optimization, Structural Analysis, and Potential Bioactivities of a Novel Polysaccharide from Sporisorium reilianum

is an important biotrophic pathogen that causes head smut disease. Polysaccharides extracted from diseased sorghum heads by exhibit significant medicinal and edible value. However, the structure and biological activities of these novel polysaccharides have not been explored. In this study, a novel p...

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Bibliographic Details
Published in:Antioxidants 2024-08, Vol.13 (8), p.965
Main Authors: Shi, He, Zhang, Siyi, Zhu, Mandi, Li, Xiaoyan, Jie, Weiguang, Kan, Lianbao
Format: Article
Language:English
Subjects:
antioxidant activity
Antioxidants
antitumor activity
Apoptosis
Cellulose
Chemical properties
Climate change
Ethanol
Experiments
Extraction (Chemistry)
Flow velocity
Food
Food consumption
Free radicals
Fruit bodies
Fungi
Health aspects
Hemodialysis
Medical research
Methods
Methylation
Microbiological research
Monosaccharides
Pharmaceuticals
Phenols
polysaccharide
Polysaccharides
Precipitation
Proteins
Reactive oxygen species
Sorghum
Sporisorium reilianum
Structure
Sulfur
Transmission electron microscopy
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Summary:is an important biotrophic pathogen that causes head smut disease. Polysaccharides extracted from diseased sorghum heads by exhibit significant medicinal and edible value. However, the structure and biological activities of these novel polysaccharides have not been explored. In this study, a novel polysaccharide (WM-NP'-60) was isolated and purified from the fruit bodies of and aimed to explore the structural characteristics and substantial antioxidant and antitumor properties of WM-NP'-60. Monosaccharide composition determination, periodate oxidation-Smith degradation, 1D/2D-NMR analysis, and methylation analysis revealed that WM-NP'-60 consisted mainly of -1,6-D-Glc , -1,3-D-Glc , and -1,3,6-D-Glc linkages. The antioxidant assays demonstrated that WM-NP'-60 exhibited great activities, including scavenging free radicals, chelating ferrous ions, and eliminating reactive oxygen species (ROS) within cells. The HepG2, SGC7901, and HCT116 cells examined by transmission electron microscopy (TEM) revealed typical apoptotic bodies. Therefore, a novel fungal polysaccharide (WM-NP'-60) was discovered, extracted, and purified in this experiment, with the aim of providing a reference for the development of a new generation of food and nutraceutical products suitable for human consumption.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox13080965