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Extraction Optimization, Structural Analysis, and Potential Bioactivities of a Novel Polysaccharide from Sporisorium reilianum
is an important biotrophic pathogen that causes head smut disease. Polysaccharides extracted from diseased sorghum heads by exhibit significant medicinal and edible value. However, the structure and biological activities of these novel polysaccharides have not been explored. In this study, a novel p...
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| Published in: | Antioxidants 2024-08, Vol.13 (8), p.965 |
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| Main Authors: | , , , , , |
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| container_issue | 8 |
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| container_title | Antioxidants |
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| creator | Shi, He Zhang, Siyi Zhu, Mandi Li, Xiaoyan Jie, Weiguang Kan, Lianbao |
| description | is an important biotrophic pathogen that causes head smut disease. Polysaccharides extracted from diseased sorghum heads by
exhibit significant medicinal and edible value. However, the structure and biological activities of these novel polysaccharides have not been explored. In this study, a novel polysaccharide (WM-NP'-60) was isolated and purified from the fruit bodies of
and aimed to explore the structural characteristics and substantial antioxidant and antitumor properties of WM-NP'-60. Monosaccharide composition determination, periodate oxidation-Smith degradation, 1D/2D-NMR analysis, and methylation analysis revealed that WM-NP'-60 consisted mainly of
-1,6-D-Glc
,
-1,3-D-Glc
, and
-1,3,6-D-Glc
linkages. The antioxidant assays demonstrated that WM-NP'-60 exhibited great activities, including scavenging free radicals, chelating ferrous ions, and eliminating reactive oxygen species (ROS) within cells. The HepG2, SGC7901, and HCT116 cells examined by transmission electron microscopy (TEM) revealed typical apoptotic bodies. Therefore, a novel fungal polysaccharide (WM-NP'-60) was discovered, extracted, and purified in this experiment, with the aim of providing a reference for the development of a new generation of food and nutraceutical products suitable for human consumption. |
| doi_str_mv | 10.3390/antiox13080965 |
| format | article |
| fullrecord | <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_e45dfa0c2c3842cc93daefe197faa3e6</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A807401418</galeid><doaj_id>oai_doaj_org_article_e45dfa0c2c3842cc93daefe197faa3e6</doaj_id><sourcerecordid>A807401418</sourcerecordid><originalsourceid>FETCH-LOGICAL-c437t-9623a3d9fd3640fa66da116c006521e306194a35c83c85cdcd57d5e6146fa8cd3</originalsourceid><addsrcrecordid>eNptksFu1DAQQCMEolXplSOyxIVDt9hx4sQntK1KW6miSIWzNYztrVdJvNjJquXAtzPbltJFjRUlGb95jsdTFG8FP5RS848wjCHeCMlbrlX9otgteaNmUpfi5ZP3nWI_5yWnSwtJ5OtiR2qhaUbsFr9PbsYESJ6BXa7G0IdfsPk4YFdjmnCcEnRsPkB3m0M-YDBY9jWOjham-FGIm9R1GIPLLHoG7Etcu44Q4gHxGlKwjvkUe3a1iilkuqeeJRe6AMPUvyleeeiy23947hXfP598Oz6bXVyenh_PL2ZYyWacaVVKkFZ7K1XFPShlQQiFnKu6FE5yJXQFssZWYlujRVs3tnZKVMpDi1buFef3XhthaVYp9JBuTYRg7gIxLQykMWDnjKtq64FjibKtSkQtLTjvhG48gHSKXJ_uXavpR-8sUjGoSFvS7ZkhXJtFXBshJP1tVZLhw4MhxZ-Ty6PpQ0bXdTC4OGUjudaiEqqpCX3_H7qMU6LzuKOaVnDV6H_UAmgHYfBxc6gbqZm3vKk42VqiDp-haFjXB4yD84HizyVgijkn5x83KbjZdKDZ7kBKePe0NI_4336TfwBiFdlh</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3097810679</pqid></control><display><type>article</type><title>Extraction Optimization, Structural Analysis, and Potential Bioactivities of a Novel Polysaccharide from Sporisorium reilianum</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Shi, He ; Zhang, Siyi ; Zhu, Mandi ; Li, Xiaoyan ; Jie, Weiguang ; Kan, Lianbao</creator><creatorcontrib>Shi, He ; Zhang, Siyi ; Zhu, Mandi ; Li, Xiaoyan ; Jie, Weiguang ; Kan, Lianbao</creatorcontrib><description>is an important biotrophic pathogen that causes head smut disease. Polysaccharides extracted from diseased sorghum heads by
exhibit significant medicinal and edible value. However, the structure and biological activities of these novel polysaccharides have not been explored. In this study, a novel polysaccharide (WM-NP'-60) was isolated and purified from the fruit bodies of
and aimed to explore the structural characteristics and substantial antioxidant and antitumor properties of WM-NP'-60. Monosaccharide composition determination, periodate oxidation-Smith degradation, 1D/2D-NMR analysis, and methylation analysis revealed that WM-NP'-60 consisted mainly of
-1,6-D-Glc
,
-1,3-D-Glc
, and
-1,3,6-D-Glc
linkages. The antioxidant assays demonstrated that WM-NP'-60 exhibited great activities, including scavenging free radicals, chelating ferrous ions, and eliminating reactive oxygen species (ROS) within cells. The HepG2, SGC7901, and HCT116 cells examined by transmission electron microscopy (TEM) revealed typical apoptotic bodies. Therefore, a novel fungal polysaccharide (WM-NP'-60) was discovered, extracted, and purified in this experiment, with the aim of providing a reference for the development of a new generation of food and nutraceutical products suitable for human consumption.</description><identifier>ISSN: 2076-3921</identifier><identifier>EISSN: 2076-3921</identifier><identifier>DOI: 10.3390/antiox13080965</identifier><identifier>PMID: 39199211</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>antioxidant activity ; Antioxidants ; antitumor activity ; Apoptosis ; Cellulose ; Chemical properties ; Climate change ; Ethanol ; Experiments ; Extraction (Chemistry) ; Flow velocity ; Food ; Food consumption ; Free radicals ; Fruit bodies ; Fungi ; Health aspects ; Hemodialysis ; Medical research ; Methods ; Methylation ; Microbiological research ; Monosaccharides ; Pharmaceuticals ; Phenols ; polysaccharide ; Polysaccharides ; Precipitation ; Proteins ; Reactive oxygen species ; Sorghum ; Sporisorium reilianum ; Structure ; Sulfur ; Transmission electron microscopy</subject><ispartof>Antioxidants, 2024-08, Vol.13 (8), p.965</ispartof><rights>COPYRIGHT 2024 MDPI AG</rights><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 by the authors. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c437t-9623a3d9fd3640fa66da116c006521e306194a35c83c85cdcd57d5e6146fa8cd3</cites><orcidid>0000-0002-6293-1738</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3097810679/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3097810679?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25751,27922,27923,37010,37011,44588,53789,53791,74896</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39199211$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, He</creatorcontrib><creatorcontrib>Zhang, Siyi</creatorcontrib><creatorcontrib>Zhu, Mandi</creatorcontrib><creatorcontrib>Li, Xiaoyan</creatorcontrib><creatorcontrib>Jie, Weiguang</creatorcontrib><creatorcontrib>Kan, Lianbao</creatorcontrib><title>Extraction Optimization, Structural Analysis, and Potential Bioactivities of a Novel Polysaccharide from Sporisorium reilianum</title><title>Antioxidants</title><addtitle>Antioxidants (Basel)</addtitle><description>is an important biotrophic pathogen that causes head smut disease. Polysaccharides extracted from diseased sorghum heads by
exhibit significant medicinal and edible value. However, the structure and biological activities of these novel polysaccharides have not been explored. In this study, a novel polysaccharide (WM-NP'-60) was isolated and purified from the fruit bodies of
and aimed to explore the structural characteristics and substantial antioxidant and antitumor properties of WM-NP'-60. Monosaccharide composition determination, periodate oxidation-Smith degradation, 1D/2D-NMR analysis, and methylation analysis revealed that WM-NP'-60 consisted mainly of
-1,6-D-Glc
,
-1,3-D-Glc
, and
-1,3,6-D-Glc
linkages. The antioxidant assays demonstrated that WM-NP'-60 exhibited great activities, including scavenging free radicals, chelating ferrous ions, and eliminating reactive oxygen species (ROS) within cells. The HepG2, SGC7901, and HCT116 cells examined by transmission electron microscopy (TEM) revealed typical apoptotic bodies. Therefore, a novel fungal polysaccharide (WM-NP'-60) was discovered, extracted, and purified in this experiment, with the aim of providing a reference for the development of a new generation of food and nutraceutical products suitable for human consumption.</description><subject>antioxidant activity</subject><subject>Antioxidants</subject><subject>antitumor activity</subject><subject>Apoptosis</subject><subject>Cellulose</subject><subject>Chemical properties</subject><subject>Climate change</subject><subject>Ethanol</subject><subject>Experiments</subject><subject>Extraction (Chemistry)</subject><subject>Flow velocity</subject><subject>Food</subject><subject>Food consumption</subject><subject>Free radicals</subject><subject>Fruit bodies</subject><subject>Fungi</subject><subject>Health aspects</subject><subject>Hemodialysis</subject><subject>Medical research</subject><subject>Methods</subject><subject>Methylation</subject><subject>Microbiological research</subject><subject>Monosaccharides</subject><subject>Pharmaceuticals</subject><subject>Phenols</subject><subject>polysaccharide</subject><subject>Polysaccharides</subject><subject>Precipitation</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Sorghum</subject><subject>Sporisorium reilianum</subject><subject>Structure</subject><subject>Sulfur</subject><subject>Transmission electron microscopy</subject><issn>2076-3921</issn><issn>2076-3921</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptksFu1DAQQCMEolXplSOyxIVDt9hx4sQntK1KW6miSIWzNYztrVdJvNjJquXAtzPbltJFjRUlGb95jsdTFG8FP5RS848wjCHeCMlbrlX9otgteaNmUpfi5ZP3nWI_5yWnSwtJ5OtiR2qhaUbsFr9PbsYESJ6BXa7G0IdfsPk4YFdjmnCcEnRsPkB3m0M-YDBY9jWOjham-FGIm9R1GIPLLHoG7Etcu44Q4gHxGlKwjvkUe3a1iilkuqeeJRe6AMPUvyleeeiy23947hXfP598Oz6bXVyenh_PL2ZYyWacaVVKkFZ7K1XFPShlQQiFnKu6FE5yJXQFssZWYlujRVs3tnZKVMpDi1buFef3XhthaVYp9JBuTYRg7gIxLQykMWDnjKtq64FjibKtSkQtLTjvhG48gHSKXJ_uXavpR-8sUjGoSFvS7ZkhXJtFXBshJP1tVZLhw4MhxZ-Ty6PpQ0bXdTC4OGUjudaiEqqpCX3_H7qMU6LzuKOaVnDV6H_UAmgHYfBxc6gbqZm3vKk42VqiDp-haFjXB4yD84HizyVgijkn5x83KbjZdKDZ7kBKePe0NI_4336TfwBiFdlh</recordid><startdate>20240808</startdate><enddate>20240808</enddate><creator>Shi, He</creator><creator>Zhang, Siyi</creator><creator>Zhu, Mandi</creator><creator>Li, Xiaoyan</creator><creator>Jie, Weiguang</creator><creator>Kan, Lianbao</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7T5</scope><scope>7TO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6293-1738</orcidid></search><sort><creationdate>20240808</creationdate><title>Extraction Optimization, Structural Analysis, and Potential Bioactivities of a Novel Polysaccharide from Sporisorium reilianum</title><author>Shi, He ; Zhang, Siyi ; Zhu, Mandi ; Li, Xiaoyan ; Jie, Weiguang ; Kan, Lianbao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-9623a3d9fd3640fa66da116c006521e306194a35c83c85cdcd57d5e6146fa8cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>antioxidant activity</topic><topic>Antioxidants</topic><topic>antitumor activity</topic><topic>Apoptosis</topic><topic>Cellulose</topic><topic>Chemical properties</topic><topic>Climate change</topic><topic>Ethanol</topic><topic>Experiments</topic><topic>Extraction (Chemistry)</topic><topic>Flow velocity</topic><topic>Food</topic><topic>Food consumption</topic><topic>Free radicals</topic><topic>Fruit bodies</topic><topic>Fungi</topic><topic>Health aspects</topic><topic>Hemodialysis</topic><topic>Medical research</topic><topic>Methods</topic><topic>Methylation</topic><topic>Microbiological research</topic><topic>Monosaccharides</topic><topic>Pharmaceuticals</topic><topic>Phenols</topic><topic>polysaccharide</topic><topic>Polysaccharides</topic><topic>Precipitation</topic><topic>Proteins</topic><topic>Reactive oxygen species</topic><topic>Sorghum</topic><topic>Sporisorium reilianum</topic><topic>Structure</topic><topic>Sulfur</topic><topic>Transmission electron microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shi, He</creatorcontrib><creatorcontrib>Zhang, Siyi</creatorcontrib><creatorcontrib>Zhu, Mandi</creatorcontrib><creatorcontrib>Li, Xiaoyan</creatorcontrib><creatorcontrib>Jie, Weiguang</creatorcontrib><creatorcontrib>Kan, Lianbao</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Antioxidants</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, He</au><au>Zhang, Siyi</au><au>Zhu, Mandi</au><au>Li, Xiaoyan</au><au>Jie, Weiguang</au><au>Kan, Lianbao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extraction Optimization, Structural Analysis, and Potential Bioactivities of a Novel Polysaccharide from Sporisorium reilianum</atitle><jtitle>Antioxidants</jtitle><addtitle>Antioxidants (Basel)</addtitle><date>2024-08-08</date><risdate>2024</risdate><volume>13</volume><issue>8</issue><spage>965</spage><pages>965-</pages><issn>2076-3921</issn><eissn>2076-3921</eissn><abstract>is an important biotrophic pathogen that causes head smut disease. Polysaccharides extracted from diseased sorghum heads by
exhibit significant medicinal and edible value. However, the structure and biological activities of these novel polysaccharides have not been explored. In this study, a novel polysaccharide (WM-NP'-60) was isolated and purified from the fruit bodies of
and aimed to explore the structural characteristics and substantial antioxidant and antitumor properties of WM-NP'-60. Monosaccharide composition determination, periodate oxidation-Smith degradation, 1D/2D-NMR analysis, and methylation analysis revealed that WM-NP'-60 consisted mainly of
-1,6-D-Glc
,
-1,3-D-Glc
, and
-1,3,6-D-Glc
linkages. The antioxidant assays demonstrated that WM-NP'-60 exhibited great activities, including scavenging free radicals, chelating ferrous ions, and eliminating reactive oxygen species (ROS) within cells. The HepG2, SGC7901, and HCT116 cells examined by transmission electron microscopy (TEM) revealed typical apoptotic bodies. Therefore, a novel fungal polysaccharide (WM-NP'-60) was discovered, extracted, and purified in this experiment, with the aim of providing a reference for the development of a new generation of food and nutraceutical products suitable for human consumption.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>39199211</pmid><doi>10.3390/antiox13080965</doi><orcidid>https://orcid.org/0000-0002-6293-1738</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Antioxidants, 2024-08, Vol.13 (8), p.965 |
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| source | Publicly Available Content Database; PubMed Central |
| subjects | antioxidant activity Antioxidants antitumor activity Apoptosis Cellulose Chemical properties Climate change Ethanol Experiments Extraction (Chemistry) Flow velocity Food Food consumption Free radicals Fruit bodies Fungi Health aspects Hemodialysis Medical research Methods Methylation Microbiological research Monosaccharides Pharmaceuticals Phenols polysaccharide Polysaccharides Precipitation Proteins Reactive oxygen species Sorghum Sporisorium reilianum Structure Sulfur Transmission electron microscopy |
| title | Extraction Optimization, Structural Analysis, and Potential Bioactivities of a Novel Polysaccharide from Sporisorium reilianum |
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