Inverse Phosphatidylcholine/Phosphatidylinositol Levels as Peripheral Biomarkers and Phosphatidylcholine/Lysophosphatidylethanolamine-Phosphatidylserine as Hippocampal Indicator of Postischemic Cognitive Impairment in Rats

• Published in: Frontiers in neuroscience 2018-12, Vol.12, p.989-989
• Main Authors: Sabogal-Guáqueta, Angelica Maria, Villamil-Ortiz, Javier Gustavo, Arias-Londoño, Julian David, Cardona-Gómez, Gloria Patricia
• Format: Article
• Language: English
• Subjects: Astrocytes; Biomarkers; Cell adhesion & migration; Cerebral blood flow; Cognitive ability; cognitive impairment; Dementia; Dementia disorders; Disease; Endothelium; Fatty acids; global ischemia; Hippocampus; Ischemia; Laboratory animals; Lecithin; Lipids; Mass spectroscopy; Membrane structure; Metabolism; Myelin; Neurodegenerative diseases; Neuroscience; Neurosciences; Neurotransmission; Parenchyma; Phosphatidylcholine; Phosphatidylinositol; Phosphatidylserine; phospholipid profile; Phospholipids; serum; Traumatic brain injury; Vascular dementia
• ISSN: 1662-4548; 1662-453X; 1662-453X
• DOI: 10.3389/fnins.2018.00989

Vascular dementia is a transversal phenomenon in different kinds of neurodegenerative diseases involving acute and chronic brain alterations. Specifically, the role of phospholipids in the pathogenesis of dementia remains unknown. In the present study, we explored phospholipid profiles a month postischemia in cognitively impaired rats. The two-vessel occlusion (2-VO) model was used to generate brain parenchyma ischemia in adult male rats confirmed by alterations in myelin, endothelium, astrocytes and inflammation mediator. A lipidomic analysis was performed via mass spectrometry in the hippocampus and serum a month postischemia. We found decreases in phospholipids (PLs) associated with neurotransmission, such as phosphatidylcholine (PC 32:0, PC 34:2, PC 36:3, PC 36:4, and PC 42:1), and increases in PLs implied in membrane structure and signaling, such as lysophosphatidylethanolamine (LPE 18:1, 20:3, and 22:6) and phosphatidylserine (PS 38:4, 36:2, and 40:4), in the hippocampus. Complementarily, PC (PC 34:2, PC 34:3, PC 38:5, and PC 36:5) and ether-PC (ePC 34:1, 34:2, 36:2, 38:2, and 38:3) decreased, while Lyso-PC (LPC 18:0, 18:1, 20:4, 20:5, and LPC 22:6) and phosphatidylinositol (PI 36:2, 38:4, 38:5, and 40:5), as neurovascular state sensors, increased in the serum. Taken together, these data suggest inverse PC/LPC-PI levels as peripheral biomarkers and inverse PC/LPE-PS as a central indicator of postischemic cognitive impairment in rats.