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A novel SWIM domain protein ZSWIM5 inhibits the malignant progression of non-small-cell lung cancer
Zinc finger SWIM-type containing 5 (ZSWIM5) is a newly discovered protein, which contains a novel zinc-chelating domain SWIM (CxCxnCxH), and is predicted to interact with DNA or proteins. Currently, the knowledge of functions of ZSWIM5 remains limited. In this study, we aimed to elucidate the biolog...
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| Published in: | Cancer management and research 2018-01, Vol.10, p.3245-3254 |
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| container_title | Cancer management and research |
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| creator | Xu, Ke Liu, Bin Ma, Yegang Xu, Baojin Xing, Xiaojing |
| description | Zinc finger SWIM-type containing 5 (ZSWIM5) is a newly discovered protein, which contains a novel zinc-chelating domain SWIM (CxCxnCxH), and is predicted to interact with DNA or proteins. Currently, the knowledge of functions of ZSWIM5 remains limited. In this study, we aimed to elucidate the biological functions of ZSWIM5 and their mechanisms.
We detected the expression of ZSWIM5 in samples from 139 cases of non-small-cell lung cancer (NSCLC) patients and six cell lines using immunohistochemistry and Western blot. Moreover, we explored the biological functions of ZSWIM5 in lung cancer cells by siRNA interference and cDNA transfection of ZSWIM5.
The results showed that compared with adjacent non-tumor lung tissues, ZSWIM5 expression was significantly decreased in NSCLC tissues (
=0.0199) and that the ZSWIM5-positive rate in non-tumor tissues (76.67%) was notably higher than that in NSCLC tissues (40.29%). ZSWIM5 expression in human normal bronchial epithelial cells was also much higher than that in lung cancer lines ( |
| doi_str_mv | 10.2147/CMAR.S174355 |
| format | article |
| fullrecord | <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_e47c7c996afd41649ebd41eeb7bdab56</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A574926967</galeid><doaj_id>oai_doaj_org_article_e47c7c996afd41649ebd41eeb7bdab56</doaj_id><sourcerecordid>A574926967</sourcerecordid><originalsourceid>FETCH-LOGICAL-c545t-22e12d53b0d288e0655f3c16a6c9d6d737aa3984544ae6aa9f2bac7e57e1d6bf3</originalsourceid><addsrcrecordid>eNptkk2LFDEQhhtR3HX15lkCXjzYY-e7-yIMg6sDuwiuIngJ6aS6J0N3MiY9C_77TTvjuiMmhwpVT72hircoXuJqQTCT71bXyy-LGywZ5fxRcY6xbEpMCXn84H1WPEtpW1WiwZQ9Lc5oRSglDJ8XZol8uIUB3XxfXyMbRu082sUwQY4_5iRHzm9c66aEpg2gUQ-u99pPM9VHSMkFj0KXZXyZcnUoDQwDGva-R0Z7A_F58aTTQ4IXx3hRfLv88HX1qbz6_HG9Wl6VhjM-lYQAJpbTtrKkrqESnHfUYKGFaaywkkqtaVMzzpgGoXXTkVYbCVwCtqLt6EWxPujaoLdqF92o4y8VtFO_EyH2SsfJmQEUMGmkaRqhO8uwYA20OQK0srW65SJrvT9o7fbtCNaAn6IeTkRPK95tVB9ulcB5uXWVBd4cBWL4uYc0qdGleTPaQ9gnRXA-rMakzujrf9Bt2EefV6UIIYxLLiv5l-p1HsD5LuR_zSyqllyyhohGzNTiP1S-FkZngofO5fxJw9tDg4khpQjd_Yy4UrPB1GwwdTRYxl893Ms9_MdR9A6c2cpz</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2224575707</pqid></control><display><type>article</type><title>A novel SWIM domain protein ZSWIM5 inhibits the malignant progression of non-small-cell lung cancer</title><source>PubMed Central (Open Access)</source><source>Taylor & Francis</source><source>Publicly Available Content Database</source><creator>Xu, Ke ; Liu, Bin ; Ma, Yegang ; Xu, Baojin ; Xing, Xiaojing</creator><creatorcontrib>Xu, Ke ; Liu, Bin ; Ma, Yegang ; Xu, Baojin ; Xing, Xiaojing</creatorcontrib><description>Zinc finger SWIM-type containing 5 (ZSWIM5) is a newly discovered protein, which contains a novel zinc-chelating domain SWIM (CxCxnCxH), and is predicted to interact with DNA or proteins. Currently, the knowledge of functions of ZSWIM5 remains limited. In this study, we aimed to elucidate the biological functions of ZSWIM5 and their mechanisms.
We detected the expression of ZSWIM5 in samples from 139 cases of non-small-cell lung cancer (NSCLC) patients and six cell lines using immunohistochemistry and Western blot. Moreover, we explored the biological functions of ZSWIM5 in lung cancer cells by siRNA interference and cDNA transfection of ZSWIM5.
The results showed that compared with adjacent non-tumor lung tissues, ZSWIM5 expression was significantly decreased in NSCLC tissues (
=0.0199) and that the ZSWIM5-positive rate in non-tumor tissues (76.67%) was notably higher than that in NSCLC tissues (40.29%). ZSWIM5 expression in human normal bronchial epithelial cells was also much higher than that in lung cancer lines (
<0.001). ZSWIM5-negative expression was significantly related to TNM stage (
<0.001), lymph node metastasis (
=0.002), and poor prognosis (
<0.001) of NSCLC patients. MTT and colony formation assays showed that ZSWIM5 could inhibit the proliferation and colony formation abilities of lung cancer cells. Meanwhile, the results of transwell and wound healing assays showed that ZSWIM5 could suppress the invasion and migration of lung cancer cells. Further investigation revealed that ZSWIM5 could downregulate cyclin D1, cyclin E, cyclin A2, MMP2, and MMP9 expression, which affected the proliferation, invasion, and migration abilities of lung cancer cells.
ZSWIM5 could inhibit the malignant progression of NSCLC by affecting the expression of cyclins and MMPs.</description><identifier>ISSN: 1179-1322</identifier><identifier>EISSN: 1179-1322</identifier><identifier>DOI: 10.2147/CMAR.S174355</identifier><identifier>PMID: 30233241</identifier><language>eng</language><publisher>New Zealand: Dove Medical Press Limited</publisher><subject>Cancer cells ; Cancer metastasis ; Care and treatment ; Cell cycle ; Chemotherapy ; Development and progression ; DNA ; DNA binding proteins ; Gastric cancer ; Genetic aspects ; Health aspects ; Immunohistochemistry ; Infection ; invasion ; Lung cancer ; migration ; Non-small cell lung cancer ; Novels ; NSCLC ; Original Research ; Physiological aspects ; Plasmids ; Polyclonal antibodies ; proliferation ; Proteins ; Surgery ; Thoracic surgery ; Tumors ; Wound care ; Wound healing ; Zinc Finger ; Zinc finger proteins</subject><ispartof>Cancer management and research, 2018-01, Vol.10, p.3245-3254</ispartof><rights>COPYRIGHT 2018 Dove Medical Press Limited</rights><rights>2018. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 Xu et al. This work is published and licensed by Dove Medical Press Limited 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c545t-22e12d53b0d288e0655f3c16a6c9d6d737aa3984544ae6aa9f2bac7e57e1d6bf3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2224575707/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2224575707?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,883,25736,27907,27908,36995,36996,44573,53774,53776,74877</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30233241$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Ke</creatorcontrib><creatorcontrib>Liu, Bin</creatorcontrib><creatorcontrib>Ma, Yegang</creatorcontrib><creatorcontrib>Xu, Baojin</creatorcontrib><creatorcontrib>Xing, Xiaojing</creatorcontrib><title>A novel SWIM domain protein ZSWIM5 inhibits the malignant progression of non-small-cell lung cancer</title><title>Cancer management and research</title><addtitle>Cancer Manag Res</addtitle><description>Zinc finger SWIM-type containing 5 (ZSWIM5) is a newly discovered protein, which contains a novel zinc-chelating domain SWIM (CxCxnCxH), and is predicted to interact with DNA or proteins. Currently, the knowledge of functions of ZSWIM5 remains limited. In this study, we aimed to elucidate the biological functions of ZSWIM5 and their mechanisms.
We detected the expression of ZSWIM5 in samples from 139 cases of non-small-cell lung cancer (NSCLC) patients and six cell lines using immunohistochemistry and Western blot. Moreover, we explored the biological functions of ZSWIM5 in lung cancer cells by siRNA interference and cDNA transfection of ZSWIM5.
The results showed that compared with adjacent non-tumor lung tissues, ZSWIM5 expression was significantly decreased in NSCLC tissues (
=0.0199) and that the ZSWIM5-positive rate in non-tumor tissues (76.67%) was notably higher than that in NSCLC tissues (40.29%). ZSWIM5 expression in human normal bronchial epithelial cells was also much higher than that in lung cancer lines (
<0.001). ZSWIM5-negative expression was significantly related to TNM stage (
<0.001), lymph node metastasis (
=0.002), and poor prognosis (
<0.001) of NSCLC patients. MTT and colony formation assays showed that ZSWIM5 could inhibit the proliferation and colony formation abilities of lung cancer cells. Meanwhile, the results of transwell and wound healing assays showed that ZSWIM5 could suppress the invasion and migration of lung cancer cells. Further investigation revealed that ZSWIM5 could downregulate cyclin D1, cyclin E, cyclin A2, MMP2, and MMP9 expression, which affected the proliferation, invasion, and migration abilities of lung cancer cells.
ZSWIM5 could inhibit the malignant progression of NSCLC by affecting the expression of cyclins and MMPs.</description><subject>Cancer cells</subject><subject>Cancer metastasis</subject><subject>Care and treatment</subject><subject>Cell cycle</subject><subject>Chemotherapy</subject><subject>Development and progression</subject><subject>DNA</subject><subject>DNA binding proteins</subject><subject>Gastric cancer</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Immunohistochemistry</subject><subject>Infection</subject><subject>invasion</subject><subject>Lung cancer</subject><subject>migration</subject><subject>Non-small cell lung cancer</subject><subject>Novels</subject><subject>NSCLC</subject><subject>Original Research</subject><subject>Physiological aspects</subject><subject>Plasmids</subject><subject>Polyclonal antibodies</subject><subject>proliferation</subject><subject>Proteins</subject><subject>Surgery</subject><subject>Thoracic surgery</subject><subject>Tumors</subject><subject>Wound care</subject><subject>Wound healing</subject><subject>Zinc Finger</subject><subject>Zinc finger proteins</subject><issn>1179-1322</issn><issn>1179-1322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkk2LFDEQhhtR3HX15lkCXjzYY-e7-yIMg6sDuwiuIngJ6aS6J0N3MiY9C_77TTvjuiMmhwpVT72hircoXuJqQTCT71bXyy-LGywZ5fxRcY6xbEpMCXn84H1WPEtpW1WiwZQ9Lc5oRSglDJ8XZol8uIUB3XxfXyMbRu082sUwQY4_5iRHzm9c66aEpg2gUQ-u99pPM9VHSMkFj0KXZXyZcnUoDQwDGva-R0Z7A_F58aTTQ4IXx3hRfLv88HX1qbz6_HG9Wl6VhjM-lYQAJpbTtrKkrqESnHfUYKGFaaywkkqtaVMzzpgGoXXTkVYbCVwCtqLt6EWxPujaoLdqF92o4y8VtFO_EyH2SsfJmQEUMGmkaRqhO8uwYA20OQK0srW65SJrvT9o7fbtCNaAn6IeTkRPK95tVB9ulcB5uXWVBd4cBWL4uYc0qdGleTPaQ9gnRXA-rMakzujrf9Bt2EefV6UIIYxLLiv5l-p1HsD5LuR_zSyqllyyhohGzNTiP1S-FkZngofO5fxJw9tDg4khpQjd_Yy4UrPB1GwwdTRYxl893Ms9_MdR9A6c2cpz</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Xu, Ke</creator><creator>Liu, Bin</creator><creator>Ma, Yegang</creator><creator>Xu, Baojin</creator><creator>Xing, Xiaojing</creator><general>Dove Medical Press Limited</general><general>Taylor & Francis Ltd</general><general>Dove Medical Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20180101</creationdate><title>A novel SWIM domain protein ZSWIM5 inhibits the malignant progression of non-small-cell lung cancer</title><author>Xu, Ke ; Liu, Bin ; Ma, Yegang ; Xu, Baojin ; Xing, Xiaojing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c545t-22e12d53b0d288e0655f3c16a6c9d6d737aa3984544ae6aa9f2bac7e57e1d6bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Cancer cells</topic><topic>Cancer metastasis</topic><topic>Care and treatment</topic><topic>Cell cycle</topic><topic>Chemotherapy</topic><topic>Development and progression</topic><topic>DNA</topic><topic>DNA binding proteins</topic><topic>Gastric cancer</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Immunohistochemistry</topic><topic>Infection</topic><topic>invasion</topic><topic>Lung cancer</topic><topic>migration</topic><topic>Non-small cell lung cancer</topic><topic>Novels</topic><topic>NSCLC</topic><topic>Original Research</topic><topic>Physiological aspects</topic><topic>Plasmids</topic><topic>Polyclonal antibodies</topic><topic>proliferation</topic><topic>Proteins</topic><topic>Surgery</topic><topic>Thoracic surgery</topic><topic>Tumors</topic><topic>Wound care</topic><topic>Wound healing</topic><topic>Zinc Finger</topic><topic>Zinc finger proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Ke</creatorcontrib><creatorcontrib>Liu, Bin</creatorcontrib><creatorcontrib>Ma, Yegang</creatorcontrib><creatorcontrib>Xu, Baojin</creatorcontrib><creatorcontrib>Xing, Xiaojing</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest research library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Cancer management and research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Ke</au><au>Liu, Bin</au><au>Ma, Yegang</au><au>Xu, Baojin</au><au>Xing, Xiaojing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel SWIM domain protein ZSWIM5 inhibits the malignant progression of non-small-cell lung cancer</atitle><jtitle>Cancer management and research</jtitle><addtitle>Cancer Manag Res</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>10</volume><spage>3245</spage><epage>3254</epage><pages>3245-3254</pages><issn>1179-1322</issn><eissn>1179-1322</eissn><abstract>Zinc finger SWIM-type containing 5 (ZSWIM5) is a newly discovered protein, which contains a novel zinc-chelating domain SWIM (CxCxnCxH), and is predicted to interact with DNA or proteins. Currently, the knowledge of functions of ZSWIM5 remains limited. In this study, we aimed to elucidate the biological functions of ZSWIM5 and their mechanisms.
We detected the expression of ZSWIM5 in samples from 139 cases of non-small-cell lung cancer (NSCLC) patients and six cell lines using immunohistochemistry and Western blot. Moreover, we explored the biological functions of ZSWIM5 in lung cancer cells by siRNA interference and cDNA transfection of ZSWIM5.
The results showed that compared with adjacent non-tumor lung tissues, ZSWIM5 expression was significantly decreased in NSCLC tissues (
=0.0199) and that the ZSWIM5-positive rate in non-tumor tissues (76.67%) was notably higher than that in NSCLC tissues (40.29%). ZSWIM5 expression in human normal bronchial epithelial cells was also much higher than that in lung cancer lines (
<0.001). ZSWIM5-negative expression was significantly related to TNM stage (
<0.001), lymph node metastasis (
=0.002), and poor prognosis (
<0.001) of NSCLC patients. MTT and colony formation assays showed that ZSWIM5 could inhibit the proliferation and colony formation abilities of lung cancer cells. Meanwhile, the results of transwell and wound healing assays showed that ZSWIM5 could suppress the invasion and migration of lung cancer cells. Further investigation revealed that ZSWIM5 could downregulate cyclin D1, cyclin E, cyclin A2, MMP2, and MMP9 expression, which affected the proliferation, invasion, and migration abilities of lung cancer cells.
ZSWIM5 could inhibit the malignant progression of NSCLC by affecting the expression of cyclins and MMPs.</abstract><cop>New Zealand</cop><pub>Dove Medical Press Limited</pub><pmid>30233241</pmid><doi>10.2147/CMAR.S174355</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cancer management and research, 2018-01, Vol.10, p.3245-3254 |
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| source | PubMed Central (Open Access); Taylor & Francis; Publicly Available Content Database |
| subjects | Cancer cells Cancer metastasis Care and treatment Cell cycle Chemotherapy Development and progression DNA DNA binding proteins Gastric cancer Genetic aspects Health aspects Immunohistochemistry Infection invasion Lung cancer migration Non-small cell lung cancer Novels NSCLC Original Research Physiological aspects Plasmids Polyclonal antibodies proliferation Proteins Surgery Thoracic surgery Tumors Wound care Wound healing Zinc Finger Zinc finger proteins |
| title | A novel SWIM domain protein ZSWIM5 inhibits the malignant progression of non-small-cell lung cancer |
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