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Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silage
Ensiling has long been as a mainstream technology of preserving forage for ruminant production. This study investigated the effects of bioaugmented ensiling with laccase and Pediococcus pentosaceus on the fermentation quality, nutritive value, enzymatic hydrolysis, and bacterial community of alfalfa...
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Published in: | Frontiers in microbiology 2022-10, Vol.13, p.1035942-1035942 |
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creator | Bao, Xueyan Feng, Haoran Guo, Gang Huo, Wenjie Li, Qinghong Xu, Qingfang Liu, Qiang Wang, Cong Chen, Lei |
description | Ensiling has long been as a mainstream technology of preserving forage for ruminant production. This study investigated the effects of bioaugmented ensiling with laccase and
Pediococcus pentosaceus
on the fermentation quality, nutritive value, enzymatic hydrolysis, and bacterial community of alfalfa. The application of laccase and
Pediococcus pentosaceus
combination was more potent in modulating the fermentation quality of silage than laccase and
Pediococcus pentosaceus
alone, as indicated by higher lactic acid contents and lactic acid to acetic acid ratios, and lower pH, dry matter losses, and ammonia nitrogen contents. Moreover, treatments with additive enhanced protein preservation and structural carbohydrate degradation, while increasing true protein and water-soluble carbohydrate contents. By promoting lignin degradation, treatments containing laccase further facilitated the release of sugars from cellulose compared with treatment with
Pediococcus pentosaceus
alone. The additive treatments reduced the bacterial diversity and optimized the bacterial community composition of silage, with an increase in the relative abundance of desirable
Lactobacillus
and a decrease in the relative abundance of undesirable
Enterobacter
and
Klebsiella
. PICRUSt functional prediction based on Kyoto Encyclopedia of Genes and Genomes (KEGG) databases revealed that PL and LPL treatments increased the metabolism of membrane transport, carbohydrate, and terpenoids and polyketides related to fermentation activities. It can be concluded that bioaugmented ensiling with laccase and
Pediococcus pentosaceus
combination can be an effective and practical strategy to improve silage fermentation and nutrient preservation of alfalfa silage. |
doi_str_mv | 10.3389/fmicb.2022.1035942 |
format | article |
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Pediococcus pentosaceus
on the fermentation quality, nutritive value, enzymatic hydrolysis, and bacterial community of alfalfa. The application of laccase and
Pediococcus pentosaceus
combination was more potent in modulating the fermentation quality of silage than laccase and
Pediococcus pentosaceus
alone, as indicated by higher lactic acid contents and lactic acid to acetic acid ratios, and lower pH, dry matter losses, and ammonia nitrogen contents. Moreover, treatments with additive enhanced protein preservation and structural carbohydrate degradation, while increasing true protein and water-soluble carbohydrate contents. By promoting lignin degradation, treatments containing laccase further facilitated the release of sugars from cellulose compared with treatment with
Pediococcus pentosaceus
alone. The additive treatments reduced the bacterial diversity and optimized the bacterial community composition of silage, with an increase in the relative abundance of desirable
Lactobacillus
and a decrease in the relative abundance of undesirable
Enterobacter
and
Klebsiella
. PICRUSt functional prediction based on Kyoto Encyclopedia of Genes and Genomes (KEGG) databases revealed that PL and LPL treatments increased the metabolism of membrane transport, carbohydrate, and terpenoids and polyketides related to fermentation activities. It can be concluded that bioaugmented ensiling with laccase and
Pediococcus pentosaceus
combination can be an effective and practical strategy to improve silage fermentation and nutrient preservation of alfalfa silage.</description><identifier>ISSN: 1664-302X</identifier><identifier>EISSN: 1664-302X</identifier><identifier>DOI: 10.3389/fmicb.2022.1035942</identifier><language>eng</language><publisher>Frontiers Media S.A</publisher><subject>alfalfa silage ; bacterial community ; bio-additives ; enzymatic hydrolysis ; fermentation quality ; Microbiology</subject><ispartof>Frontiers in microbiology, 2022-10, Vol.13, p.1035942-1035942</ispartof><rights>Copyright © 2022 Bao, Feng, Guo, Huo, Li, Xu, Liu, Wang and Chen. 2022 Bao, Feng, Guo, Huo, Li, Xu, Liu, Wang and Chen</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-6f1a83f086b755343de96ccc6a03c007ba2010e43dbf5172457fb36c532fec83</citedby><cites>FETCH-LOGICAL-c445t-6f1a83f086b755343de96ccc6a03c007ba2010e43dbf5172457fb36c532fec83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582240/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582240/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Bao, Xueyan</creatorcontrib><creatorcontrib>Feng, Haoran</creatorcontrib><creatorcontrib>Guo, Gang</creatorcontrib><creatorcontrib>Huo, Wenjie</creatorcontrib><creatorcontrib>Li, Qinghong</creatorcontrib><creatorcontrib>Xu, Qingfang</creatorcontrib><creatorcontrib>Liu, Qiang</creatorcontrib><creatorcontrib>Wang, Cong</creatorcontrib><creatorcontrib>Chen, Lei</creatorcontrib><title>Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silage</title><title>Frontiers in microbiology</title><description>Ensiling has long been as a mainstream technology of preserving forage for ruminant production. This study investigated the effects of bioaugmented ensiling with laccase and
Pediococcus pentosaceus
on the fermentation quality, nutritive value, enzymatic hydrolysis, and bacterial community of alfalfa. The application of laccase and
Pediococcus pentosaceus
combination was more potent in modulating the fermentation quality of silage than laccase and
Pediococcus pentosaceus
alone, as indicated by higher lactic acid contents and lactic acid to acetic acid ratios, and lower pH, dry matter losses, and ammonia nitrogen contents. Moreover, treatments with additive enhanced protein preservation and structural carbohydrate degradation, while increasing true protein and water-soluble carbohydrate contents. By promoting lignin degradation, treatments containing laccase further facilitated the release of sugars from cellulose compared with treatment with
Pediococcus pentosaceus
alone. The additive treatments reduced the bacterial diversity and optimized the bacterial community composition of silage, with an increase in the relative abundance of desirable
Lactobacillus
and a decrease in the relative abundance of undesirable
Enterobacter
and
Klebsiella
. PICRUSt functional prediction based on Kyoto Encyclopedia of Genes and Genomes (KEGG) databases revealed that PL and LPL treatments increased the metabolism of membrane transport, carbohydrate, and terpenoids and polyketides related to fermentation activities. It can be concluded that bioaugmented ensiling with laccase and
Pediococcus pentosaceus
combination can be an effective and practical strategy to improve silage fermentation and nutrient preservation of alfalfa silage.</description><subject>alfalfa silage</subject><subject>bacterial community</subject><subject>bio-additives</subject><subject>enzymatic hydrolysis</subject><subject>fermentation quality</subject><subject>Microbiology</subject><issn>1664-302X</issn><issn>1664-302X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVks2KFDEQxxtRcFn3BTzl6GFmzGd_XARZVl1Y8LIHb6GSrsxkSXdmk26hfRif1fTMKG4ISeVflV-lQlXVe0Z3QrTdRzd4a3accr5jVKhO8lfVFatruRWU_3j9n_22usn5iZYhKS_rVfX7zjm0UybRkQDWQkYCY7_ak7cErO-JKTYmDySOZDogcZgGHCeYfBGeZwh-WjZknKfki0xsHI4x-9W7ITj-WgZYUYelTzEs2efNKcNfalgvDPNYIOsjILh1kuwD7PFd9aYcM95c9uvq8cvd4-237cP3r_e3nx-2Vko1bWvHoBWOtrVplBJS9NjV1toaqLCUNgY4ZRSLbpxiDZeqcUbUVgleqm_FdXV_xvYRnvQx-QHSoiN4fRJi2mtIpYiAGmUnlOwKm4NsXd81DUXDsDMGeidoYX06s46zGbC35UsShBfQl57RH_Q-_tSdajmXK-DDBZDi84x50oPPFkOAEeOcNW94y2TdMVVC-TnUpphzQvcvDaN67Q196g299oa-9Ib4AyiistE</recordid><startdate>20221006</startdate><enddate>20221006</enddate><creator>Bao, Xueyan</creator><creator>Feng, Haoran</creator><creator>Guo, Gang</creator><creator>Huo, Wenjie</creator><creator>Li, Qinghong</creator><creator>Xu, Qingfang</creator><creator>Liu, Qiang</creator><creator>Wang, Cong</creator><creator>Chen, Lei</creator><general>Frontiers Media S.A</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20221006</creationdate><title>Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silage</title><author>Bao, Xueyan ; Feng, Haoran ; Guo, Gang ; Huo, Wenjie ; Li, Qinghong ; Xu, Qingfang ; Liu, Qiang ; Wang, Cong ; Chen, Lei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-6f1a83f086b755343de96ccc6a03c007ba2010e43dbf5172457fb36c532fec83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>alfalfa silage</topic><topic>bacterial community</topic><topic>bio-additives</topic><topic>enzymatic hydrolysis</topic><topic>fermentation quality</topic><topic>Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bao, Xueyan</creatorcontrib><creatorcontrib>Feng, Haoran</creatorcontrib><creatorcontrib>Guo, Gang</creatorcontrib><creatorcontrib>Huo, Wenjie</creatorcontrib><creatorcontrib>Li, Qinghong</creatorcontrib><creatorcontrib>Xu, Qingfang</creatorcontrib><creatorcontrib>Liu, Qiang</creatorcontrib><creatorcontrib>Wang, Cong</creatorcontrib><creatorcontrib>Chen, Lei</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Open Access: DOAJ - Directory of Open Access Journals</collection><jtitle>Frontiers in microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bao, Xueyan</au><au>Feng, Haoran</au><au>Guo, Gang</au><au>Huo, Wenjie</au><au>Li, Qinghong</au><au>Xu, Qingfang</au><au>Liu, Qiang</au><au>Wang, Cong</au><au>Chen, Lei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silage</atitle><jtitle>Frontiers in microbiology</jtitle><date>2022-10-06</date><risdate>2022</risdate><volume>13</volume><spage>1035942</spage><epage>1035942</epage><pages>1035942-1035942</pages><issn>1664-302X</issn><eissn>1664-302X</eissn><abstract>Ensiling has long been as a mainstream technology of preserving forage for ruminant production. This study investigated the effects of bioaugmented ensiling with laccase and
Pediococcus pentosaceus
on the fermentation quality, nutritive value, enzymatic hydrolysis, and bacterial community of alfalfa. The application of laccase and
Pediococcus pentosaceus
combination was more potent in modulating the fermentation quality of silage than laccase and
Pediococcus pentosaceus
alone, as indicated by higher lactic acid contents and lactic acid to acetic acid ratios, and lower pH, dry matter losses, and ammonia nitrogen contents. Moreover, treatments with additive enhanced protein preservation and structural carbohydrate degradation, while increasing true protein and water-soluble carbohydrate contents. By promoting lignin degradation, treatments containing laccase further facilitated the release of sugars from cellulose compared with treatment with
Pediococcus pentosaceus
alone. The additive treatments reduced the bacterial diversity and optimized the bacterial community composition of silage, with an increase in the relative abundance of desirable
Lactobacillus
and a decrease in the relative abundance of undesirable
Enterobacter
and
Klebsiella
. PICRUSt functional prediction based on Kyoto Encyclopedia of Genes and Genomes (KEGG) databases revealed that PL and LPL treatments increased the metabolism of membrane transport, carbohydrate, and terpenoids and polyketides related to fermentation activities. It can be concluded that bioaugmented ensiling with laccase and
Pediococcus pentosaceus
combination can be an effective and practical strategy to improve silage fermentation and nutrient preservation of alfalfa silage.</abstract><pub>Frontiers Media S.A</pub><doi>10.3389/fmicb.2022.1035942</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | alfalfa silage bacterial community bio-additives enzymatic hydrolysis fermentation quality Microbiology |
title | Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silage |
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