Complementary methods for SARS-CoV-2 diagnosis in times of material shortage

The pandemic caused by SARS-CoV-2 resulted in increasing demands for diagnostic tests, leading to a shortage of recommended testing materials and reagents. This study reports on the performance of self-sampled alternative swabbing material (ordinary Q-tips tested against flocked swab and rayon swab)...

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Bibliographic Details
Published in:Scientific reports 2021-06, Vol.11 (1), p.11899-11899, Article 11899
Main Authors: Sandri, Thaisa Lucas, Inoue, Juliana, Geiger, Johanna, Griesbaum, Johanna-Marie, Heinzel, Constanze, Burnet, Michael, Fendel, Rolf, Kremsner, Peter G., Held, Jana, Kreidenweiss, Andrea
Format: Article
Language:English
Subjects:
631/326/2521
631/337
692/699/255
COVID-19
Dyes
Guanidine
Humanities and Social Sciences
multidisciplinary
Pandemics
Phenols
Reagents
Reverse transcription
Science
Science (multidisciplinary)
Severe acute respiratory syndrome coronavirus 2
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Summary:The pandemic caused by SARS-CoV-2 resulted in increasing demands for diagnostic tests, leading to a shortage of recommended testing materials and reagents. This study reports on the performance of self-sampled alternative swabbing material (ordinary Q-tips tested against flocked swab and rayon swab), of reagents for classical RNA extraction (phenol/guanidine-based protocol against a commercial kit), and of intercalating dye-based one-step quantitative reverse transcription real-time PCRs (RT-qPCR) compared against the gold standard hydrolysis probe-based assays for SARS-CoV-2 detection. The study found sampling with Q-tips, RNA extraction with classical protocol and intercalating dye-based RT-qPCR as a reliable and comparably sensitive strategy for detection of SARS-CoV-2—particularly valuable in the current period with a resurgent and dramatic increase in SARS-CoV-2 infections and growing shortage of diagnostic materials especially for regions limited in resources.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-91457-z