Wheat Bran Extract Regulates Mast Cell-Mediated Allergic Responses In Vitro and In Vivo

• Published in: Molecules (Basel, Switzerland) Switzerland), 2020-09, Vol.25 (17), p.3997
• Main Authors: Lee, Jae Yeon, Ahn, Eun-Kyung, Park, Ju-Hyoung, Oh, Joa Sub
• Format: Article
• Language: English
• Subjects: Acids; Allergens; Allergic diseases; Allergic rhinitis; Allergies; allergy; Anaphylaxis; Animals; Antigens; Antigens - immunology; Atopic dermatitis; beta-N-Acetylhexosaminidases - metabolism; Cell Degranulation - drug effects; Cell Line; Cell Survival - drug effects; Compound 48/80; Cyclooxygenase-2; Cytokines; Degranulation; Dermatitis; Dietary Fiber - pharmacology; Enzymes; Extracellular signal-regulated kinase; Food allergies; Histamine; Hypersensitivity - pathology; Immunoglobulin E; Immunoglobulin E - metabolism; In vivo methods and tests; Inactivation; Inflammation; Inflammation Mediators - metabolism; Kinases; MAP kinase; MAP Kinase Signaling System - drug effects; Mast Cells - drug effects; Mast Cells - pathology; Mast Cells - physiology; Mice, Inbred BALB C; mitogen-activated protein kinase; Molecular modelling; Mortality; Nitric oxide; Nitric-oxide synthase; p-Methoxy-N-methylphenethylamine - pharmacology; Passive Cutaneous Anaphylaxis - drug effects; Plant Extracts - pharmacology; Prebiotics; Protein kinase; Proteins; Rats, Sprague-Dawley; Rhinitis; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Wheat; Wheat bran
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25173997

In the present study the effects and molecular mechanisms of wheat bran (WB), the hard outer layer of the wheat kernel used in food ingredients, on mast cell-mediated allergic responses in vitro and in vivo were investigated. The water extract of WB inhibited degranulation and expression of allergic and inflammatory mediators such as tumor necrosis factor-α, cyclooxygenase-2 and inducible nitric oxide synthase in antigen-stimulated RBL-2H3 cells. These anti-allergic activities of WB were mediated by the inactivation of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase, which play important roles in degranulation and expression of various allergic and inflammatory molecules. In agreement with its in vitro effects, WB inhibited immunoglobulin E (IgE)/antigen-induced and compound 48/80-induced anaphylactic reactions in vivo. Taken together, these findings suggest the pharmacological potential of WB in the regulation of allergic diseases, including allergic rhinitis, atopic dermatitis, asthma and anaphylaxis.