Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells

Mechanical forces are pervasive in the inflammatory site where dendritic cells (DCs) are activated to migrate into draining lymph nodes. For example, fluid shear stress modulates the movement patterns of DCs, including directness and forward migration indices (FMIs), without chemokine effects. Howev...

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Bibliographic Details
Published in:Scientific reports 2021-06, Vol.11 (1), p.12106-12106, Article 12106
Main Authors: Kang, Ji-Hun, Lee, Hyun Joo, Kim, Ok-Hyeon, Yun, Yong Ju, Seo, Young-Jin, Lee, Hyun Jung
Format: Article
Language:English
Subjects:
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Biomechanics
Bone marrow
CD86 antigen
Cell activation
Chemokines
Computer applications
Dendritic cells
Edema
Fluid flow
Humanities and Social Sciences
Inflammation
Leukocyte migration
Lymph nodes
Mechanical stimuli
Microfluidics
multidisciplinary
Science
Science (multidisciplinary)
Shear stress
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Summary:Mechanical forces are pervasive in the inflammatory site where dendritic cells (DCs) are activated to migrate into draining lymph nodes. For example, fluid shear stress modulates the movement patterns of DCs, including directness and forward migration indices (FMIs), without chemokine effects. However, little is known about the effects of biomechanical forces on the activation of DCs. Accordingly, here we fabricated a microfluidics system to assess how biomechanical forces affect the migration and activity of DCs during inflammation. Based on the structure of edema, we proposed and experimentally analyzed a novel concept for a microchip model that mimicked such vascular architecture. The intensity of shear stress generated in our engineered chip was found as 0.2–0.6 dyne/cm 2 by computational simulation; this value corresponded to inflammation in tissues. In this platform, the directness and FMIs of DCs were significantly increased, whereas the migration velocity of DCs was not altered by shear stress, indicating that mechanical stimuli influenced DC migration. Moreover, DCs with shear stress showed increased expression of the DC activation markers MHC class I and CD86 compared with DCs under static conditions. Taken together, these data suggest that the biomechanical forces are important to regulate the migration and activity of DCs.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-91117-2