Establishment and characterization of induced pluripotent stem cells from placental mesenchymal stromal cells

Human term placenta is a bulky organ which harbours abundant mesenchymal stromal cells. This study reports isolation and characterization of placental mesenchymal stromal cells (PMSCs) followed by reprogramming of PMSCs to induced pluripotent stem cells (iPSCs). The placental human iPSC (PhiPSC) lin...

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Bibliographic Details
Published in:Stem cell research 2018-03, Vol.27, p.15-20
Main Author: Parveen, Shagufta
Format: Article
Language:English
Subjects:
Cell Differentiation - physiology
Cells, Cultured
Female
Human term placenta
Humans
Induced Pluripotent Stem Cells - cytology
Karyotype
Karyotypically normal
Mesenchymal Stem Cells - cytology
Placenta - cytology
Placental human induced pluripotent stem cells
Placental mesenchymal stromal cells
Pregnancy
Teratoma
Teratoma - metabolism
Transgene free
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Summary:Human term placenta is a bulky organ which harbours abundant mesenchymal stromal cells. This study reports isolation and characterization of placental mesenchymal stromal cells (PMSCs) followed by reprogramming of PMSCs to induced pluripotent stem cells (iPSCs). The placental human iPSC (PhiPSC) line is pluripotent with high telomerase activity, genetically identical to parental PMSCs, transgene free, karyotypically normal and differentiates into ectoderm, mesoderm and endoderm both in vitro and in vivo. Thus PMSCs serve as a unique cell type for human perinatal iPSC derivation from extra embryonic tissue.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2017.12.008