Integrated Analysis of Long Non-Coding RNA Expression Profiles in Glaesserella parasuis-Induced Meningitis: New Insight into Pathogenesis

• Published in: Microbiology research 2023-09, Vol.14 (3), p.1427-1441
• Main Authors: Sun, Peiyan, Yang, Yaqiong, Cheng, Hongxing, Fu, Shulin, Liu, Yulan, Qiu, Yinsheng, Chen, Hongbo, Zhang, Jing, Zhou, Huanhuan, Shi, Liangyu, Ren, Hongyan, Chao, Zhe, Guo, Ling
• Format: Article
• Language: English
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Animals; Brain; Cell adhesion; Cell adhesion molecules; Data analysis; Gene expression; Genes; Genomes; Glaesserella parasuis; Hogs; Infections; Inflammation; Laboratories; lncRNAs; Meningitis; Metastasis; Nitrogen; Non-coding RNA; Ontology; Pathogenesis; porcine brain; Protein interaction; Proteins; Signal transduction; Software; Swine; Tumorigenesis
• ISSN: 2036-7481; 2036-7481
• DOI: 10.3390/microbiolres14030097

Glaesserella parasuis (G. parasuis) can elicit meningitis in pigs; however, the pathogenic mechanisms of meningitis induced by G. parasuis remain unclear. Long non-coding RNAs (lncRNAs) have been proven to play key roles in a variety of physiological and pathological processes. However, whether lncRNAs are involved in meningitis triggered by G. parasuis has not been investigated. In this study, we performed an integrative analysis of lncRNAs expression profiles in the porcine brain infected with G. parasuis using RNA-seq. The results showed that lncRNA expressions in G. parasuis-induced meningitis were modified, and a total of 306 lncRNAs exhibited significant differential expression, in which 176 lncRNAs were up-regulated and 130 lncRNAs were down-regulated. KEGG enrichment analysis demonstrated that the differentially expressed target mRNAs of affected lncRNAs in G. parasuis-infected porcine brain were mainly involved in the cell adhesion molecules (CAMs), Jak-STAT signaling pathway, PI3k-Akt signaling pathway, and TNF signaling pathway. The expression relationship between the most affected differential lncRNAs and their differential target mRNAs was visualized by a co-expression network. A protein-protein interaction network consisting of 12 differential targets was constructed using STRING analysis. In addition, differential expressions of important lncRNAs were validated by qRT-PCR. lncRNA ALDBSSCT0000007362, ALDBSSCT0000001959, ALDBSSCT0000005529, MSTRG.2939.1, and MSTRG.32374.1 showed the same expression pattern with the lncRNA sequencing data. Our results demonstrated that G. parasuis could modify the lncRNA expression profiles in the porcine brain. To the best of our knowledge, this is the first report revealing the integrative analysis of lncRNA expression profiles in G. parasuis-induced meningitis, which could enhance important information to understand the inflammatory functions of lncRNAs involved in swine meningitis, and also provide a foundation for finding out novel strategies to prevent and treat meningitis in piglets triggered by G. parasuis.