Molecular diagnosis for detecting KRAS mutation in peritoneal washing fluid of pancreatic ductal adenocarcinoma

Positive peritoneal washing cytology is an indicator of poor prognosis in patients with pancreatic ductal adenocarcinoma (PDAC); however, its sensitivity is relatively low. This study evaluated the performance of peptide nucleic acid (PNA)-directed PCR clamping as a molecular-based peritoneal washin...

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Bibliographic Details
Published in:Scientific reports 2024-09, Vol.14 (1), p.21732-12, Article 21732
Main Authors: Shimane, Gaku, Nakano, Yutaka, Matsuda, Sachiko, Kitago, Minoru, Masugi, Yohei, Nakamura, Kohei, Nakamura, Yuki, Yagi, Hiroshi, Abe, Yuta, Hasegawa, Yasushi, Hori, Shutaro, Tanaka, Masayuki, Takemura, Ryo, Nishihara, Hiroshi, Kitagawa, Yuko
Format: Article
Language:English
Subjects:
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Adenocarcinoma
Adult
Aged
Aged, 80 and over
Ascitic Fluid - pathology
Carcinoma, Pancreatic Ductal - diagnosis
Carcinoma, Pancreatic Ductal - genetics
Carcinoma, Pancreatic Ductal - pathology
Cellular biology
Cytology
Diagnosis
Female
Humanities and Social Sciences
Humans
KRAS
Male
Middle Aged
multidisciplinary
Mutation
Nucleic acids
Pancreas
Pancreatic cancer
Pancreatic ductal adenocarcinoma
Pancreatic Neoplasms - diagnosis
Pancreatic Neoplasms - genetics
Pancreatic Neoplasms - pathology
Peptide nucleic acids
Peritoneal Lavage
Peritoneal washing fluid
Peritoneum
Polymerase chain reaction
Proto-Oncogene Proteins p21(ras) - genetics
ROC Curve
Science
Science (multidisciplinary)
Sensitivity analysis
Sensitivity and Specificity
Citations: Items that this one cites
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Summary:Positive peritoneal washing cytology is an indicator of poor prognosis in patients with pancreatic ductal adenocarcinoma (PDAC); however, its sensitivity is relatively low. This study evaluated the performance of peptide nucleic acid (PNA)-directed PCR clamping as a molecular-based peritoneal washing cytology for sensitive detection of KRAS mutation in PDAC. Intraoperative peritoneal washing fluid (IPWF) obtained from patients with PDAC who underwent surgery was analyzed. PNA-directed PCR clamping was performed on DNA extracted from IPWF. Among 54 patients enrolled, threshold cycle (Ct) was significantly lower in patients with positive peritoneal washing cytology than in those with negative peritoneal washing cytology ( P  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-72569-8