Osteogenic assessment of leukocyte platelet-rich fibrin and injectable platelet-rich fibrin in the human osteosarcoma MG - 63 cell line in chronic periodontitis patients: An in vitro study

The burgeoning interest in implant and regenerative dental care has led to a notable upsurge in the utilization of regenerative modalities. The intent of the present investigation was to evaluate the osteogenic ability of two different concentrated platelet groups at various concentrations in the hu...

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Published in:Journal of Indian Society of Periodontology 2024-03, Vol.28 (2), p.192-196
Main Authors: Dolly, A Sheryl, Rajula, Prem Blaisie, Shankar, P L Ravi, Lochini, S, Rashik, Mohamed, Raja, Sindhujaa
Format: Article
Language:English
Subjects:
Bone cancer
Bone implants
Bone morphogenetic protein 2
Cell culture
Cell differentiation
Fibrin
Gum disease
Leukocytes
Original
Osteocalcin
osteogenic potential
Osteosarcoma
Periodontitis
platelet concentrates
Platelets
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Summary:The burgeoning interest in implant and regenerative dental care has led to a notable upsurge in the utilization of regenerative modalities. The intent of the present investigation was to evaluate the osteogenic ability of two different concentrated platelet groups at various concentrations in the human osteosarcoma MG-63 cell line. Blood samples from 21 volunteers with chronic periodontitis were collected which were then centrifuged in accordance with the protocols of Choukroun . and Miron . to produce leukocyte- and platelet-rich fibrin (L-PRF) and injectable platelet-rich fibrin (I-PRF), respectively. Following MG-63 cell culture, the osteogenic ability of 0, 4%, and 20% concentrations of both L-PRF and I-PRF were determined using the real-time polymerase chain reaction assay. The results showed that 20% I-PRF (1.52 ± 0.24) and 4% L-PRF (1.42 ± 0.37) had the highest amount of bone morphogenetic protein 2 and osteocalcin, respectively. I-PRF appears to promote the initial differentiation of cells.
ISSN:0972-124X
0975-1580
DOI:10.4103/jisp.jisp_503_23