Transcriptome Analysis of the Influence of High-Pressure Carbon Dioxide on Saccharomyces cerevisiae under Sub-Lethal Condition

High-pressure carbon dioxide (HPCD), a novel non-thermal pasteurization technology, has attracted the attention of scientists due to its high pasteurization efficiency at a lower temperature and pressure. However, the inactivation mechanism has not been well researched, and this has hindered its com...

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Bibliographic Details
Published in:Journal of fungi (Basel) 2022-09, Vol.8 (10), p.1011
Main Authors: Yu, Tonghuan, Takahashi, Ukyo, Iwahashi, Hitoshi
Format: Article
Language:English
Subjects:
Apoptosis
Carbon dioxide
Cell death
cell membrane
Cell walls
Gene deletion
Gene expression
Genomes
Glutathione
high-pressure carbon dioxide
Lecithin
ontology analysis
Pasteurization
Pentose phosphate pathway
Phosphatidylcholine
Pressure
Protein biosynthesis
Protein folding
Protein turnover
Protoplasts
RNA sequencing
Saccharomyces cerevisiae
Software
Sterilization
transcriptome analysis
Transcriptomes
Yeast
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Summary:High-pressure carbon dioxide (HPCD), a novel non-thermal pasteurization technology, has attracted the attention of scientists due to its high pasteurization efficiency at a lower temperature and pressure. However, the inactivation mechanism has not been well researched, and this has hindered its commercial application. In this work, we used a sub-lethal HPCD condition (4.0 MPa, 30 °C) and a recovery condition (30 °C) to repair the damaged cells. Transcriptome analysis was performed by using RNA sequencing and gene ontology analysis to investigate the detailed lethal mechanism caused by HPCD treatment. RT-qPCR analysis was conducted for certain upregulated genes, and the influence of HPCD on protoplasts and single-gene deletion strains was investigated. Six major categories of upregulated genes were identified, including genes associated with the pentose phosphate pathway (oxidative phase), cell wall organization or biogenesis, glutathione metabolism, protein refolding, phosphatidylcholine biosynthesis, and AdoMet synthesis, which are all considered to be associated with cell death induced by HPCD. The inactivation or structure alteration of YNL194Cp in the organelle membrane is considered the critical reason for cell death. We believe this work contributes to elucidating the cell-death mechanism and providing a direction for further research on non-thermal HPCD sterilization technology.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof8101011