MiR-133b Affect the Proliferation and Drug Sensitivity in A549 Lung Cancer Stem Cells by Targeting PKM2

• Published in: Zhongguo fei ai za zhi 2017-06, Vol.20 (6), p.376-381
• Main Authors: Mi, Yonghua, He, Miao, Liu, Beizhong
• Format: Article
• Language: Chinese
• Subjects: A549; A549 Cells; Apoptosis - drug effects; Binding sites; Cell growth; Cell Proliferation - drug effects; Cisplatin - pharmacology; DDP; Gene Expression Regulation, Neoplastic - drug effects; Humans; Lung cancer; Lung Neoplasms - drug therapy; Lung Neoplasms - enzymology; Lung Neoplasms - genetics; Lung Neoplasms - physiopathology; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; MiR-133b; Neoplastic Stem Cells - drug effects; Neoplastic Stem Cells - enzymology; PKM2; Pyruvate Kinase - antagonists & inhibitors; Pyruvate Kinase - genetics; Pyruvate Kinase - metabolism; Stem cells
• ISSN: 1009-3419; 1999-6187
• DOI: 10.3779/j.issn.1009-3419.2017.06.02

It has been proven that miR-133b could inhibit cancer cell growth, the expression level of miR-133b was significant reduction in lung cancer tissue and serum of patients, and increase the radiation sensitivity of squamous cell carcinoma by targeting PKM2, but the exist mechanisms is not clear. The aim of this study is to explore the effect of miR-133b on proliferation in A549 lung cancer stem cells and drug sensitivity in DDP, and to explore the relationship between miR-133b and PKM2 gene, as well as the effect of cancer stem cells. Using miRBase and miRNAMap database to sequence comparison miR-133b and PKM2 gene. Using immune magnetic separation method to select the CD133+/CD34+ lung cancer stem cells from A549 cells, and using flow cytometry to detect the purity. The expression of miR-133b mRNA was detected by real-time fluorescence quantitative PCR (qRT-PCR). Cell proliferation was detected by CCK8 assay. 15 μg/mL DDP was treated to cells which was transfected with miR-133b, and apoptosis was detected by f