Phytochemical Characterization of Taxus baccata L. Aril with Emphasis on Evaluation of the Antiproliferative and Pro-Apoptotic Activity of Rhodoxanthin

Taxus baccata L., an evergreen tree, was known until recently due to its high concentration of toxic compounds. The purpose of the present study was to focus on the only non-poisonous part, the red arils, which have recently been described as an important source of various bioactive constituents. To...

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Bibliographic Details
Published in:Antioxidants 2022-05, Vol.11 (6), p.1039
Main Authors: Dumitraş, Daria-Antonia, Bunea, Andrea, Vodnar, Dan Cristian, Hanganu, Daniela, Pall, Emoke, Cenariu, Mihai, Gal, Adrian Florin, Andrei, Sanda
Format: Article
Language:English
Subjects:
Acetic acid
antioxidant activity
Antioxidants
Apoptosis
Asthma
Birds
Carotenoids
Cell lines
Chromatography
Column chromatography
Cytotoxicity
Endangered & extinct species
Ethyl acetate
Flavonoids
High-performance liquid chromatography
Hydrogen peroxide
Keratinocytes
Melanoma
Oxidative stress
Petroleum ether
Phenolic compounds
Pigments
Poisoning
rhodoxanthin
Seeds
Taxus baccata
Taxus baccata L. arils
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Summary:Taxus baccata L., an evergreen tree, was known until recently due to its high concentration of toxic compounds. The purpose of the present study was to focus on the only non-poisonous part, the red arils, which have recently been described as an important source of various bioactive constituents. To establish total phenolic, flavonoid, and carotenoid content, antioxidant capacity, and cytotoxic properties, two types of extracts were obtained. The chemical profile of the ethanolic extract was evaluated using chromatographic (HPLC-DAD-ESI+) and spectral (UV-Vis) methods, and the antioxidant activity of the ethanolic extract was assessed using DPPH and FRAP assays, yielding moderate results. In the second type of extract (methanol: ethyl acetate: petroleum ether (1:1:1, v/v/v)) we identified three carotenoids using open column chromatography and RP–PAD–HPLC, with rhodoxanthin being the most abundant. Considering the above and mainly because of the lack of information in the literature about this pigment and its biological effects, we decided to further investigate the cytotoxic activity of rhodoxanthin, the main carotenoid presented in aril, and its protective effect against H2O2-induced oxidative stress using two cell lines: human HaCaT keratinocytes and B16F10 murine malignant melanoma. The MTT and Annexin-V Apoptosis assays showed a substantial cytotoxic potential expressed in a dose-dependent manner towards the melanoma cell line, however, no obvious cytotoxic effects on human keratinocytes were noticed.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox11061039