miR3633a-GA3ox2 Module Conducts Grape Seed-Embryo Abortion in Response to Gibberellin

• Published in: International journal of molecular sciences 2022-08, Vol.23 (15), p.8767
• Main Authors: Bai, Yunhe, Zhang, Xiaowen, Xuan, Xuxian, Sadeghnezhad, Ehsan, Liu, Fei, Dong, Tianyu, Pei, Dan, Fang, Jinggui, Wang, Chen
• Format: Article
• Language: English
• Subjects: Abortion; Amino acids; Berries; Biosynthesis; embryo abortion; Embryos; Enzymatic activity; Enzymes; Flowers & plants; Fruits; GA3ox2; Genes; Genetic transformation; gibberellin; Gibberellins; Grapes; grapevine; Grapevines; In vivo methods and tests; Metabolism; miR3633a; Phylogenetics; Physiology; Proteins; RNA-mediated interference; Seeds; Signal transduction; Vitaceae
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23158767

Seedlessness is one of the important quality and economic traits favored by grapevine consumers, which are mainly affected by phytohormones, especially gibberellin (GA). GA is widely utilized in seedless berry production and could effectively induce grape seed embryo abortion. However, the molecular mechanism underlying this process, like the role of RNA silencing in the biosynthesis pathway of GA remains elusive. Here, Gibberellin 3-β dioxygenase2 (GA3ox2) as the last key enzyme in GA biosynthesis was predicated as a potential target gene for miR3633a, and two of them were identified as a GA response in grape berries. We also analyzed the promoter regions of genes encoding GA biosynthesis and found the hormone-responsive elements to regulate grape growth and development. The cleavage interaction between VvmiR3633a and VvGA3ox2 was validated by RLM-RACE and the transient co-transformation technique in tobacco in vivo. Interestingly, during GA-induced grape seed embryo abortion, exogenous GA promoted the expression of VvmiR3633a, thereby mainly repressing the level of VvGA3ox2 in seed embryos. We also observed a negative correlation between down-regulated VvGA20ox2/VvGA3ox2 and up-regulated VvGA2ox3/VvGA2ox1, of which GA inactivation was greater than GA synthesis, inhibited active GA content, accompanied by the reduction of VvSOD and VvCAT expression levels and enzymatic activities. These series of changes might be the main causes of grape seed embryo abortion. In conclusion, we have preliminarily drawn a schematic mode of GA-mediated VvmiR3633a and related genes regulatory network during grape seed abortion induced by exogenous GA. Our findings provide novel insights into the GA-responsive roles of the VvmiR3633a-VvGA3ox2 module in the modulation of grape seed-embryo abortion, which has implications for the molecular breeding of high-quality seedless grape berries.